BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20026

Title: Mutagenesis and nuclear magnetic resonance analyses of the fusion peptide of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus F protein   PubMed: 18524820

Authors: Tan, Y.; Jiang, L.; Wang, M.L.; Yin, F.F.; Deng, F.; Liu, M.L.; Hu, Z.H.; Wang, H.L.

Citation: Tan, Y.; Jiang, L.; Wang, M.L.; Yin, F.F.; Deng, F.; Liu, M.L.; Hu, Z.H.; Wang, H.L.. "Mutagenesis and nuclear magnetic resonance analyses of the fusion peptide of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus F protein"  J. Virol. 82, 8138-8148 (2008).

Assembly members:
F protein, polymer, 20 residues, 2144.487 Da.

Natural source:   Common Name: cotton bollworm   Taxonomy ID: 29058   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Helicoverpa armigera

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
F protein: NIGLNFVGSVDKFLFGVMDA

Data sets:
Data typeCount
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1F protein1

Entities:

Entity 1, F protein 20 residues - 2144.487 Da.

1   ASNILEGLYLEUASNPHEVALGLYSERVAL
2   ASPLYSPHELEUPHEGLYVALMETASPALA

Samples:

sample_1: entity 2 mM; sodium phosphate 20 mM; SDS, [U-100% 2H], 200 mM

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

DBJ BAG74706
GB AAG53876 AAK96377 AAL56143 ABI35801 AEN04056
REF NP_075202 NP_203690 NP_542760 YP_002274064 YP_009051827