BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20025

Title: NMR solution structure of mu-conotoxin SIIIA   PubMed: 18522941

Authors: Lewis, Richard; Schroeder, Christina

Citation: Schroeder, Christina; Ekberg, Jenny; Nielsen, Katherine; Adams, Denise; Loughnan, Marion; Thomas, Linda; Adams, David; Alewood, Paul; Lewis, Richard. "Neuronally Selective {micro}-Conotoxins from Conus striatus Utilize an {alpha}-Helical Motif to Target Mammalian Sodium Channels"  J. Biol. Chem. 283, 21621-21628 (2008).

Assembly members:
SIIIA, polymer, 20 residues, 2348.551 Da.

Natural source:   Common Name: Striated cone   Taxonomy ID: 6493   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus striatus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SIIIA: XNCCNGGCSSKWCRDHARCC

Data sets:
Data typeCount
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SIIIA1

Entities:

Entity 1, SIIIA 20 residues - 2348.551 Da.

1   GLPASNCYSCYSASNGLYGLYCYSSERSER
2   LYSTRPCYSARGASPHISALAARGCYSCYS

Samples:

sample_1: SIIIA 2 mM

sample_2: SIIIA 2 mM

sample_conditions_1: pH: 3; temperature: 286.5 K

sample_conditions_2: pH: 3; temperature: 276.5 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H COSYsample_2isotropicsample_conditions_2

Software:

UXNMR, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment, peak picking

DYANA, Guntert, Braun and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

    Related Database Links:

    BMRB 20023
    GB AAO48588 AEX60326 AEX60330 AEX60428
    SP Q86DU6