BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20014

Title: STRUCTURE OF ANTIBACTERIAL PEPTIDE CAPISTRUIN: A 19-RESIDUE LARIAT PROTOKNOT WITH A THREADED 9-MEMBER SIDECHAIN TO-BACKBONE RING   PubMed: 18671394

Authors: Knappe, T.; Linne, U.; Zirah, S.; Rebuffat, S.; Xie, X.; Marahi, M.

Citation: Knappe, T.; Linne, U.; Zirah, S.; Rebuffat, S.; Xie, X.; Marahiel, M.. "Isolation and structural characterization of capistruin, a lasso peptide predicted from the genome sequence of Burkholderia thailandensis E264"  J. Am. Chem. Soc. 130, 11446-11454 (2008).

Assembly members:
CAPISTRUIN, polymer, 19 residues, 2051.26 Da.

Natural source:   Common Name: Burkholderia thailandensis   Taxonomy ID: 57975   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia thailandensis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CAPISTRUIN: GTPGFQTPDARVISRFGFN

Data sets:
Data typeCount
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CAPISTRUIN1

Entities:

Entity 1, CAPISTRUIN 19 residues - 2051.26 Da.

1   GLYTHRPROGLYPHEGLNTHRPROASPALA
2   ARGVALILESERARGPHEGLYPHEASN

Samples:

sample_1: CAPISTRUIN 8.6 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0 mM; pH: .; pressure: 1 bar; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, G NTERT, P. - geometry optimization

TOPSPIN v2.1, Bruker Biospin - processing

SPARKY v2.1, Goddard - data analysis

NMR spectrometers:

  • BRUKER AVANCE 600 MHz

Related Database Links:

EMBL CFB48912 CFD83320 CFD86202 CFK34393 CFK38857
GB ABN83845 ABN91987 ACQ98583 AFI66377 AGK46793