BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20011

Title: NMR STRUCTURE OF THE MUTANT OF CHIGNOLIN, CLN025   PubMed: 18950166

Authors: KATO, Yusuke; ISHIMURA, Miyuki; HONDA, Shinya

Citation: Honda, Shinya; Akiba, Toshihiko; Kato, Yusuke; Sawada, Yoshito; Sekijima, Masakazu; Ishimura, Miyuki; Ooishi, Ayako; Watanabe, Hideki; Odahara, Takayuki; Harata, Kazuaki. "Crystal structure of a ten-amino acid protein"  J. Am. Chem. Soc. 130, 15327-15331 (2008).

Assembly members:
CLN025, polymer, 10 residues, 1294.336 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CLN025: YYDPETGTWY

Data sets:
Data typeCount
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CLN0251

Entities:

Entity 1, CLN025 10 residues - 1294.336 Da.

Mutant of Cignolin, CLN025

1   TYRTYRASPPROGLUTHRGLYTHRTRPTYR

Samples:

sample_1: CLN025 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; DSS 0.01%; H2O 95%; D2O 5%

sample_conditions_1: pH: 5.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

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