BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20010

Title: Superimposed fifteen structures of 17-residue peptide corresponding to the segment within ice nucleation protein of X. campestris pv. campestris   PubMed: 18361918

Authors: Kumaki, Yasuhiro; Kawano, Keiichi; Hikichi, Kunio; Matsumoto, Takeshi; Matsushima, Norio

Citation: Kumaki, Yasuhiro; Kawano, Keiichi; Hikichi, Kunio; Matsumoto, Takeshi; Matsushima, Norio. "A circular loop of the 16-residue repeating unit in ice nucleation protein"  Biochem. Biophys. Res. Commun. 371, 5-9 (2008).

Assembly members:
model_peptide_for_INP, polymer, 19 residues, 1964.123 Da.

Natural source:   Common Name: Xanthomonas campestris   Taxonomy ID: 339   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Xanthomonas campestris

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
model_peptide_for_INP: XQTARKGSDLTTGYGSTSX

Data sets:
Data typeCount
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1model_peptide_for_INP1

Entities:

Entity 1, model_peptide_for_INP 19 residues - 1964.123 Da.

1   ACEGLNTHRALAARGLYSGLYSERASPLEU
2   THRTHRGLYTYRGLYSERTHRSERNH2

Samples:

sample_1: model peptide for INP 3 mM; DSS 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 4.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • JEOL ALPHA 500 MHz

Related Database Links:

DBJ GAE51334 GAE56226 GAE61386
EMBL CAP49871 CEM56732
GB AAM39823 AAY47600 AEL08859 AKC80693 AKU51266
REF NP_635899 WP_002804985 WP_006450271 WP_011035756 WP_014509183