BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19730

Title: Structural Mapping of a Chaperone Substrate Interaction Surface   PubMed: 24700611

Authors: Callon, Morgane; Burmann, Bjoern; Hiller, Sebastian

Citation: Callon, Morgane; Burmann, Bjorn; Hiller, Sebastian. "Structural Mapping of a Chaperone-Substrate Interaction Surface."  Angew. Chem. Int. Ed. Engl. ., .-. (2014).

Assembly members:
Escherichia_Coli_Skp, polymer, 162 residues, Formula weight is not available
Escherichia_Coli_tOmpA, polymer, 177 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Escherichia_Coli_Skp: MGSSHHHHHHSSGLVPRGSH MADKIAIVNMGSLFQQVAQK TGVSNTLENEFKGRASELQR METDLQAKMKKLQSMKAGSD RTKLEKDVMAQRQTFAQKAQ AFEQDRARRSNEERGKLVTR IQTAVKSVANSQDIDLVVDA NAVAYNSSDVKDITADVLKQ VK
Escherichia_Coli_tOmpA: MAPKDNTWYTGAKLGWSQYH DTGFINNNGPTHENQLGAGA FGGYQVNPYVGFEMGYDWLG RMPYKGSVENGAYKAQGVQL TAKLGYPITDDLDIYTRLGG MVWRADTKSNVYGKNHDTGV SPVFAGGVEYAITPEIATRL EYQWTNNIGDAHTIGTRPDN GMLSLGVSYRFGQGEAA

Data sets:
Data typeCount
13C chemical shifts13
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Skp, chain 11
2Skp, chain 21
3Skp, chain 31
4tOmpA2

Entities:

Entity 1, Skp, chain 1 162 residues - Formula weight is not available

native processed E. coli Skp (residues 21 - 161) hexahistidin-tag and thrombin cleavage site

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALAASPLYSILEALAILEVALASNMET
4   GLYSERLEUPHEGLNGLNVALALAGLNLYS
5   THRGLYVALSERASNTHRLEUGLUASNGLU
6   PHELYSGLYARGALASERGLULEUGLNARG
7   METGLUTHRASPLEUGLNALALYSMETLYS
8   LYSLEUGLNSERMETLYSALAGLYSERASP
9   ARGTHRLYSLEUGLULYSASPVALMETALA
10   GLNARGGLNTHRPHEALAGLNLYSALAGLN
11   ALAPHEGLUGLNASPARGALAARGARGSER
12   ASNGLUGLUARGGLYLYSLEUVALTHRARG
13   ILEGLNTHRALAVALLYSSERVALALAASN
14   SERGLNASPILEASPLEUVALVALASPALA
15   ASNALAVALALATYRASNSERSERASPVAL
16   LYSASPILETHRALAASPVALLEULYSGLN
17   VALLYS

Entity 2, tOmpA 177 residues - Formula weight is not available

native processed E. Coli OmpA (residues 22-197) + initiator methionine

1   METALAPROLYSASPASNTHRTRPTYRTHR
2   GLYALALYSLEUGLYTRPSERGLNTYRHIS
3   ASPTHRGLYPHEILEASNASNASNGLYPRO
4   THRHISGLUASNGLNLEUGLYALAGLYALA
5   PHEGLYGLYTYRGLNVALASNPROTYRVAL
6   GLYPHEGLUMETGLYTYRASPTRPLEUGLY
7   ARGMETPROTYRLYSGLYSERVALGLUASN
8   GLYALATYRLYSALAGLNGLYVALGLNLEU
9   THRALALYSLEUGLYTYRPROILETHRASP
10   ASPLEUASPILETYRTHRARGLEUGLYGLY
11   METVALTRPARGALAASPTHRLYSSERASN
12   VALTYRGLYLYSASNHISASPTHRGLYVAL
13   SERPROVALPHEALAGLYGLYVALGLUTYR
14   ALAILETHRPROGLUILEALATHRARGLEU
15   GLUTYRGLNTRPTHRASNASNILEGLYASP
16   ALAHISTHRILEGLYTHRARGPROASPASN
17   GLYMETLEUSERLEUGLYVALSERTYRARG
18   PHEGLYGLNGLYGLUALAALA

Samples:

sample_1: Escherichia_Coli_Skp (trimer), [U-13CE, U-1H]-Met; [U-13CB; U-1HB]-Ala; [U-2H; U-15N], 0.7 mM; H2O 90%; D2O 10%; MES 25 mM; NaCl 150 mM; Escherichia_Coli_tOmpA, [U-13CD, U-1HD]-Ile; [U-13CG; U-1HG]-Leu; [U-13CG; U-1HG]_Val; [U-2H; U-15N], 0.4 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

XEASY, Keller and Wuthrich - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection

PROSA, Guntert - processing

NMR spectrometers:

  • Bruker AscendII 700 MHz

Related Database Links:

PDB
BMRB 19409 19410 19407 19407 19408 19409 19733 19409 19410 19940
UNP P0AEU7 P0A910
DBJ BAA77853 BAB33603 BAG75701 BAI23539 BAI29054 BAA35715 BAB34464 BAG76542 BAI24400 BAI29850
EMBL CAA38567 CAP74747 CAQ30692 CAQ97065 CAR01553 CAA23588 CAP75420 CAQ31485 CAR02310 CAR07182
GB AAA24630 AAB08607 AAC73289 AAG54480 AAN41830 AAC74043 AAF37887 AAG55443 AAP74759 AAT02227
REF NP_308207 NP_414720 NP_706123 WP_000758954 WP_000758955 NP_286832 NP_309068 NP_415477 WP_000091488 WP_000315442
SP P0AEU7 P0AEU8 P0AEU9 P0AEV0 Q325W2 P0A910 P0A911