BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19672

Title: NMR Characterization of the Near Native and Unfolded States of the PTB Domain of Dok1: Alternate Conformations and Residual Clusters   PubMed: 24587391

Authors: Gupta, Sebanti

Citation: Gupta, Sebanti; Bhattacharjya, Surajit. "NMR characterization of the near native and unfolded states of the PTB domain of Dok1: alternate conformations and residual clusters."  PLoS ONE 9, e90557-e90557 (2014).

Assembly members:
Dok1_PTB_domain, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Dok1_PTB_domain: WVTVQRTEAAERCGLHGSYV LRVEAERLTLLTVGAQSQIL EPLLSWPYTLLRRYGRDKVM FSFEAGRRCPSGPGTFTFQT AQGNDIFQAVETAIHRQKAQ G

Data sets:
Data typeCount
13C chemical shifts95
15N chemical shifts86
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dok1 PTB domain monomer1

Entities:

Entity 1, Dok1 PTB domain monomer 101 residues - Formula weight is not available

1   TRPVALTHRVALGLNARGTHRGLUALAALA
2   GLUARGCYSGLYLEUHISGLYSERTYRVAL
3   LEUARGVALGLUALAGLUARGLEUTHRLEU
4   LEUTHRVALGLYALAGLNSERGLNILELEU
5   GLUPROLEULEUSERTRPPROTYRTHRLEU
6   LEUARGARGTYRGLYARGASPLYSVALMET
7   PHESERPHEGLUALAGLYARGARGCYSPRO
8   SERGLYPROGLYTHRPHETHRPHEGLNTHR
9   ALAGLNGLYASNASPILEPHEGLNALAVAL
10   GLUTHRALAILEHISARGGLNLYSALAGLN
11   GLY

Samples:

sample_1: Dok1 PTB domain, [U-13C; U-15N], 0.4 – 0.6 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15551
PDB
DBJ BAD96603 BAG51595 BAG58893 BAJ17765
GB AAB88182 AAC51127 AAI14441 AAX93224 AIC48651
REF NP_001184189 NP_001372 XP_001111248 XP_001161802 XP_002757635
SP Q99704