BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19656

Title: CD79b cytosolic domain phosphorylated, denatured state   PubMed: 23667548

Authors: Rosenlow, Joakim

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Rosenlow, Joakim; Brutscher, Bernhard; Karlsson, B. Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains."  PLoS ONE 8, e62947-e62947 (2013).

Assembly members:
CD79b_cyt_phosp, polymer, 51 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
CD79b_cyt_phosp: SLLDKDDSKAGMEEDHTXEG LDIDQTATXEDIVTLRTGEV KWSVGEHPGQE

Data sets:
Data typeCount
13C chemical shifts138
15N chemical shifts47
1H chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD79b_cyt_phosp1

Entities:

Entity 1, CD79b_cyt_phosp 51 residues - Formula weight is not available

1   SERLEULEUASPLYSASPASPSERLYSALA
2   GLYMETGLUGLUASPHISTHRPTRGLUGLY
3   LEUASPILEASPGLNTHRALATHRPTRGLU
4   ASPILEVALTHRLEUARGTHRGLYGLUVAL
5   LYSTRPSERVALGLYGLUHISPROGLYGLN
6   GLU

Samples:

CD79b_cytosolic_domain_phosphorylated_denatured: CD79b_cyt_phosp, [U-99% 13C; U-99% 15N], 200 – 300 uM; DTT 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; Complete EDTA-free 1 X; TSP 100 uM; magnesium chloride 12 mM; ATP 1 mM; Fyn kinase 300 nM; sodium chloride 150 mM; urea 6 M; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.000; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1
3D HN(ca)COCD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1
3D HNCACD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1
3D HNCACBCD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1
3D HNCOCD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1
3D HN(CO)CACD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1
3D HN(COCA)CBCD79b_cytosolic_domain_phosphorylated_denaturedisotropicsample_conditions_1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - Spectrum analysis

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing

TANSY v1.0, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

  • Varian Unity Inova 600 MHz

Related Database Links:

UniProt P40259
BMRB 19655