BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19651

Title: CD79b cytosolic domain denatured   PubMed: 23667548

Authors: Rosenlow, Joakim

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Rosenlow, Joakim; Brutscher, Bernhard; Karlsson, B. Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains."  PLoS ONE 8, e62947-e62947 (2013).

Assembly members:
CD79b_cyt, polymer, 51 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
CD79b_cyt: SLLDKDDSKAGMEEDHTYEG LDIDQTATYEDIVTLRTGEV KWSVGEHPGQE

Data sets:
Data typeCount
13C chemical shifts139
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD79b_cyt1

Entities:

Entity 1, CD79b_cyt 51 residues - Formula weight is not available

1   SERLEULEUASPLYSASPASPSERLYSALA
2   GLYMETGLUGLUASPHISTHRTYRGLUGLY
3   LEUASPILEASPGLNTHRALATHRTYRGLU
4   ASPILEVALTHRLEUARGTHRGLYGLUVAL
5   LYSTRPSERVALGLYGLUHISPROGLYGLN
6   GLU

Samples:

CD79b_cytosolic_domain_denatured: CD79b_cyt, [U-99% 13C; U-99% 15N], 200-300 uM; DTT 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; Complete EDTA-free 1 X; TSP 100 uM; sodium chloride 150 mM; urea 6 M; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.000; pressure: 1.000 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCD79b_cytosolic_domain_denaturedisotropicsample_conditions_1
3D HN(ca)COCD79b_cytosolic_domain_denaturedisotropicsample_conditions_1
3D HNCACD79b_cytosolic_domain_denaturedisotropicsample_conditions_1
3D HNCACBCD79b_cytosolic_domain_denaturedisotropicsample_conditions_1
3D HNCOCD79b_cytosolic_domain_denaturedisotropicsample_conditions_1
3D HN(CO)CACD79b_cytosolic_domain_denaturedisotropicsample_conditions_1
3D HN(COCA)CBCD79b_cytosolic_domain_denaturedisotropicsample_conditions_1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - Spectrum analysis

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing

TANSY v1.0, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

  • Varian Unity Inova 600 MHz

Related Database Links:

UniProt P40259
BMRB 18884 19650
DBJ BAA25652 BAD96674 BAD96930 BAG36353
EMBL CAA58522
GB AAA58387 AAA64459 AAA72424 AAB24822 AAC60654
REF NP_000617 NP_001035022 NP_001230841 NP_001248454 NP_067613
SP P40259
TPG DAA18410