BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19181

Title: 1H, and 15N Chemical Shift Assignments for mycoloylated PorH in DMSO solution   PubMed: 24100136

Authors: Saurel, Olivier; Demange, Pascal; Rath, Parth; Tropis, Maryelle; Daffe, Mamadou; MILON, Alain

Citation: Rath, Parthasarathi; Saurel, Olivier; Tropis, Maryelle; Daffe, Mamadou; Demange, Pascal; Milon, Alain. "NMR localization of the O-mycoloylation on PorH, a channel forming peptide from Corynebacterium glutamicum."  FEBS Lett. 587, 3687-3691 (2013).

Assembly members:
PorH, polymer, 74 residues, Formula weight is not available

Natural source:   Common Name: High GC Gram+   Taxonomy ID: 1718   Superkingdom: Bacteria   Kingdom: not available   Genus/species: corynebacterium glutamicum

Experimental source:   Production method: recombinant technology   Host organism: C. glutamicum PorA PorH

Entity Sequences (FASTA):
PorH: MDLSLLKETLGNYETFGGNI GTALQSIPTLLDSILNFFDN FGDLADTTGENLDNFSSIEG RASRGSHHHHHHHH

Data sets:
Data typeCount
15N chemical shifts66
1H chemical shifts351

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PorH1

Entities:

Entity 1, PorH 74 residues - Formula weight is not available

1   METASPLEUSERLEULEULYSGLUTHRLEU
2   GLYASNTYRGLUTHRPHEGLYGLYASNILE
3   GLYTHRALALEUGLNSERILEPROTHRLEU
4   LEUASPSERILELEUASNPHEPHEASPASN
5   PHEGLYASPLEUALAASPTHRTHRGLYGLU
6   ASNLEUASPASNPHESERSERILEGLUGLY
7   ARGALASERARGGLYSERHISHISHISHIS
8   HISHISHISHIS

Samples:

sample_1: PorH, [U-99% 15N], 1 mM; mycolic acid 1 mM; DMSO 100%

sample_conditions_1: temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

EMBL CAF20738 CAI39424
GB AGT06429