BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19134

Title: Backbone assignments of 8M urea-denatured YbeA from Escherichia coli   PubMed: 23821130

Authors: Hsieh, Shu-Ju Micky; Hsu, Shang-Te Danny

Citation: Hsieh, Shu-Ju Micky; Mallam, Anna; Jackson, Sophie; Hsu, Shang-Te Danny. "Backbone NMR assignments of a topologically knotted protein in urea-denatured state."  Biomol. NMR Assign. 8, 283-285 (2014).

Assembly members:
YbeA, polymer, 155 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
YbeA: MKLQLVAVGTKMPDWVQTGF TEYLRRFPKDMPFELIEIPA GKRGKNADIKRILDKEGEQM LAAAGKNRIVTLDIPGKPWD TPQLAAELERWKLDGRDVSL LIGGPEGLSPACKAAAEQSW SLSALTLPHPLVRVLVAESL YRAWSITTNHPYHRE

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts135
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YbeA1

Entities:

Entity 1, YbeA 155 residues - Formula weight is not available

1   METLYSLEUGLNLEUVALALAVALGLYTHR
2   LYSMETPROASPTRPVALGLNTHRGLYPHE
3   THRGLUTYRLEUARGARGPHEPROLYSASP
4   METPROPHEGLULEUILEGLUILEPROALA
5   GLYLYSARGGLYLYSASNALAASPILELYS
6   ARGILELEUASPLYSGLUGLYGLUGLNMET
7   LEUALAALAALAGLYLYSASNARGILEVAL
8   THRLEUASPILEPROGLYLYSPROTRPASP
9   THRPROGLNLEUALAALAGLULEUGLUARG
10   TRPLYSLEUASPGLYARGASPVALSERLEU
11   LEUILEGLYGLYPROGLUGLYLEUSERPRO
12   ALACYSLYSALAALAALAGLUGLNSERTRP
13   SERLEUSERALALEUTHRLEUPROHISPRO
14   LEUVALARGVALLEUVALALAGLUSERLEU
15   TYRARGALATRPSERILETHRTHRASNHIS
16   PROTYRHISARGGLU

Samples:

sample_1: YbeA, [U-13C; U-15N], 350 uM; D2O 10%; H2O 90%; TRIS 50 mM; potassium chloride 200 mM; glycerol 10%; urea 7.2 M

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAA35283 BAB34097 BAG76228 BAI24039 BAI29506
EMBL CAA28200 CAD05118 CAP75135 CAQ31111 CAQ89966
GB AAB40836 AAC73737 AAG54970 AAL19592 AAN42281
PIR AD0581
REF NP_308701 NP_415169 NP_455217 NP_459633 NP_706574
SP A1A8R1 A4W822 A7ZJ25 A7ZXR2 A8AJG7