BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19115

Title: Full-length mercury transporter protein MerF in lipid bilayer membranes   PubMed: 23763519

Authors: Lu, George; Tian, Ye; Vora, Nemil; Marassi, Francesca; Opella, Stanley

Citation: Lu, George; Tian, Ye; Vora, Nemil; Marassi, Francesca; Opella, Stanley. "The Structure of the Mercury Transporter MerF in Phospholipid Bilayers: A Large Conformational Rearrangement Results from N-Terminal Truncation"  J. Am. Chem. Soc. 135, 9299-9302 (2013).

Assembly members:
MerF, polymer, 81 residues, 8670.492 Da.

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 582   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Morganella morganii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MerF: MKDPKTLLRVSIIGTTLVAL SSFTPVLVILLGVVGLSALT GYLDYVLLPALAIFIGLTIY AIQRKRQADASSTPKFNGVK K

Data sets:
Data typeCount
13C chemical shifts116
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MerF1

Entities:

Entity 1, MerF 81 residues - 8670.492 Da.

Residue 81 Z is homoserine resulting from cyanogen bromide cleavage

1   METLYSASPPROLYSTHRLEULEUARGVAL
2   SERILEILEGLYTHRTHRLEUVALALALEU
3   SERSERPHETHRPROVALLEUVALILELEU
4   LEUGLYVALVALGLYLEUSERALALEUTHR
5   GLYTYRLEUASPTYRVALLEULEUPROALA
6   LEUALAILEPHEILEGLYLEUTHRILETYR
7   ALAILEGLNARGLYSARGGLNALAASPALA
8   SERSERTHRPROLYSPHEASNGLYVALLYS
9   LYS

Samples:

sample_1: MerF, [U-99% 13C; U-99% 15N], 2.0 – 3.0 mg; 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine10.0 – 15.0 mg; MES 20 mM; H2O 100%

sample_conditions_1: pH: 6.0; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1solidsample_conditions_1
2D NC correlationsample_1solidsample_conditions_1
3D HnNCa SLFsample_1solidsample_conditions_1
3D HnNCo SLFsample_1solidsample_conditions_1
3D HcCxCx SLFsample_1solidsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian DD2 800 MHz

Related Database Links:

BMRB 17914
PDB