BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18867

Title: CD79a cytosolic domain   PubMed: 23667548

Authors: Mayzel, Maxim

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains"  PLoS One 8, e62947-e62947 (2013).

Assembly members:
CD79a, polymer, 63 residues, 7188.7093 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: Wheat germ - cell freeE. coli - cell free

Entity Sequences (FASTA):
CD79a: SLRKRWQNEKLGLDAGDEYE DENLYEGLNLDDCSMYEDIS RGLQGTYQDVGSLNIGDVQL EKP

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts120
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD79a, native1
2CD79a, denatured1

Entities:

Entity 1, CD79a, native 63 residues - 7188.7093 Da.

1   SERLEUARGLYSARGTRPGLNASNGLULYS
2   LEUGLYLEUASPALAGLYASPGLUTYRGLU
3   ASPGLUASNLEUTYRGLUGLYLEUASNLEU
4   ASPASPCYSSERMETTYRGLUASPILESER
5   ARGGLYLEUGLNGLYTHRTYRGLNASPVAL
6   GLYSERLEUASNILEGLYASPVALGLNLEU
7   GLULYSPRO

Samples:

Nativ: CD79a, [U-99% 13C; U-99% 15N], 0.1 ± 1e-05 mM; DTT 2 mM; EDTA 1 mM; potassium phosphate 20 mM; azide 0.02%; Complete EDTA-free 1 x; H2O 90%; D2O 10%

Denatured: CD79a, [U-99% 13C; U-99% 15N], 0.1 mM; DTT 2 mM; EDTA 1 mM; potassium phosphate 20 mM; azide 0.02%; Complete EDTA-free 1 x; UREA 6 M; H2O 90%; D2O 10%

Normal: ionic strength: 0.020 M; pH: 6.700; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNativisotropicNormal
3D HN(ca)CONativisotropicNormal
3D HNCANativisotropicNormal
3D HNCACBNativisotropicNormal
3D HNCONativisotropicNormal
3D HN(CO)CANativisotropicNormal
3D HN(COCA)CBNativisotropicNormal
2D 1H-15N HSQCDenaturedisotropicNormal
3D HN(ca)CODenaturedisotropicNormal
3D HNCADenaturedisotropicNormal
3D HNCACBDenaturedisotropicNormal
3D HNCODenaturedisotropicNormal
3D HN(CO)CADenaturedisotropicNormal
3D HN(COCA)CBDenaturedisotropicNormal

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - Spectrum analysis

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing

TANSY v1.0, (MDDNMR) Mayzel, Orekhov - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

BMRB 19644 19645 19648 19649 19723 19724
DBJ BAG37737
EMBL CAA31802 CAA58523
GB AAA20495 AAA57274 AAA59556 AAA59557 AAA60270
REF NP_001774 NP_067612 XP_001152742 XP_002829327 XP_003779238
SP P11912