BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18855

Title: Backbone assignments of 8M urea denatured YibK from Haemophilus influenzae (HI0766)   PubMed: 23975348

Authors: Hsieh, Shu-Ju Micky; Hsu, Shang-Te Danny

Citation: Hsieh, Shu-Ju Micky; Mallam, Anna; Jackson, Sophie; Hsu, Shang-Te Danny. "Erratum to: Backbone 1H, 13C and 15N assignments of YibK and a variant containing a unique cysteine residue at C-terminus in 8 M urea-denatured states."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
YibK, polymer, 160 residues, Formula weight is not available

Natural source:   Common Name: Haemophilus influenzae   Taxonomy ID: 727   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
YibK: MLDIVLYEPEIPQNTGNIIR LCANTGFRLHLIEPLGFTWD DKRLRRSGLDYHEFAEIKRH KTFEAFLESEKPKRLFALTT KGCPAHSQVKFKLGDYLMFG PETRGIPMSILNEMPMEQKI RIPMTANSRSMNLSNSVAVT VYEAWRQLGYKGAVNLPEVK

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts146
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YibK1

Entities:

Entity 1, YibK 160 residues - Formula weight is not available

1   METLEUASPILEVALLEUTYRGLUPROGLU
2   ILEPROGLNASNTHRGLYASNILEILEARG
3   LEUCYSALAASNTHRGLYPHEARGLEUHIS
4   LEUILEGLUPROLEUGLYPHETHRTRPASP
5   ASPLYSARGLEUARGARGSERGLYLEUASP
6   TYRHISGLUPHEALAGLUILELYSARGHIS
7   LYSTHRPHEGLUALAPHELEUGLUSERGLU
8   LYSPROLYSARGLEUPHEALALEUTHRTHR
9   LYSGLYCYSPROALAHISSERGLNVALLYS
10   PHELYSLEUGLYASPTYRLEUMETPHEGLY
11   PROGLUTHRARGGLYILEPROMETSERILE
12   LEUASNGLUMETPROMETGLUGLNLYSILE
13   ARGILEPROMETTHRALAASNSERARGSER
14   METASNLEUSERASNSERVALALAVALTHR
15   VALTYRGLUALATRPARGGLNLEUGLYTYR
16   LYSGLYALAVALASNLEUPROGLUVALLYS

Samples:

YibK_in_denatured_state: YibK, [U-13C; U-15N], 350 uM; D2O 10%; TRIS 50 mM; potassium chloride 200 mM; glycerol 10%; urea 7.2 M; H2O 90%

SEC-urea: pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCYibK_in_denatured_stateisotropicSEC-urea
3D CBCA(CO)NHYibK_in_denatured_stateisotropicSEC-urea
3D HNCOYibK_in_denatured_stateisotropicSEC-urea
3D HNCACBYibK_in_denatured_stateisotropicSEC-urea
3D HNCAYibK_in_denatured_stateisotropicSEC-urea
3D C(CO)NHYibK_in_denatured_stateisotropicSEC-urea

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CBW29084 CBY81616 CBY86097 CKG76086
GB AAC22424 AAX87814 ABR00326 ADO81557 ADO96939
REF NP_438925 WP_005625919 WP_005636234 WP_005644428 WP_005648463
SP P44868