BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18260

Title: 1H, 13C and 15N resonance assignment of chicken brain alpha spectrin repeat 17   PubMed: 22569754

Authors: Brenner, Annette; Raae, Arnt

Citation: Brenner, Annette; Kieffer, Bruno; Trave, Gilles; Frystein, Nils Age; Raae, Arnt. "Thermal stability of chicken brain -spectrin repeat 17: a spectroscopic study."  J. Biomol. NMR 53, 71-83 (2012).

Assembly members:
R17, polymer, 118 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
R17: MARGQRLEESLEYQQFVANV EEEEAWINEKMTLVASEDYG DTLAAIQGLLKKHEAFETDF TVHKDRVNDVCANGEDLIKK NNHHVENITAKMKGLKGKVS DLEKAAAQRKAKLDENSA

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts122
1H chemical shifts770
T1 relaxation values97
T2 relaxation values97
coupling constants94
heteronuclear NOE values94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1R171

Entities:

Entity 1, R17 118 residues - Formula weight is not available

Thew first residue is a remain of the TEV cleavage site

1   METALAARGGLYGLNARGLEUGLUGLUSER
2   LEUGLUTYRGLNGLNPHEVALALAASNVAL
3   GLUGLUGLUGLUALATRPILEASNGLULYS
4   METTHRLEUVALALASERGLUASPTYRGLY
5   ASPTHRLEUALAALAILEGLNGLYLEULEU
6   LYSLYSHISGLUALAPHEGLUTHRASPPHE
7   THRVALHISLYSASPARGVALASNASPVAL
8   CYSALAASNGLYGLUASPLEUILELYSLYS
9   ASNASNHISHISVALGLUASNILETHRALA
10   LYSMETLYSGLYLEULYSGLYLYSVALSER
11   ASPLEUGLULYSALAALAALAGLNARGLYS
12   ALALYSLEUASPGLUASNSERALA

Samples:

sample_1: R17, [U-98% 13C; U-98% 15N], 0.2 ± 0.02 mM; sodium chloride 0.1 mM; DTT 0.05 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, CCPN, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P07751