BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18052

Title: Structural analysis of the pyroglutamate modified isoform of the Alzheimer's disease related beta-amyloid using NMR spectroscopy   PubMed: 23001756

Authors: Sun, Na; Hartmann, Rudolf; Lecher, Justin; Stoldt, Matthias; Willbold, Dieter

Citation: Sun, Na; Hartmann, Rudolf; Lecher, Justin; Stoldt, Matthias; Funke, Susanne Aileen; Gremer, Lothar; Ludwig, Hans-Henning; Demuth, Hans-Ulrich; Kleinschmi, Martin; Willbold, Dieter. "Structural analysis of the pyroglutamate modified isoform of the Alzheimer's disease related beta-amyloid using NMR spectroscopy"  J. Pept. Sci. 18, 691-695 (2012).

Assembly members:
Pyroglutamate_Abeta, polymer, 38 residues, 4125.6626 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis   Host organism: Synthetic

Entity Sequences (FASTA):
Pyroglutamate_Abeta: XFRHDSGYEVHHQKLVFFAE DVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
1H chemical shifts217

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PyroAb1

Entities:

Entity 1, PyroAb 38 residues - 4125.6626 Da.

1   PCAPHEARGHISASPSERGLYTYRGLUVAL
2   HISHISGLNLYSLEUVALPHEPHEALAGLU
3   ASPVALGLYSERASNLYSGLYALAILEILE
4   GLYLEUMETVALGLYGLYVALVAL

Samples:

1: Pyroglutamate Abeta 0.1 mM; Trifluoroethanol 40%; H2O 60%

Standard: ionic strength: 0.050 M; pH: 2.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSY1isotropicStandard
2D 1H-1H NOESY1isotropicStandard

Software:

ANALYSIS v2.1, CCPN - assignment

NMRPipe v5.99.2011.242.16.23, NIH - conversion, processing

VnmrJ v2.3A, Agilent Technologies (formerly Varian) - data recording, spectrometer operation

NMR spectrometers:

  • Varian VNMRS 900 MHz

Related Database Links:

BMRB 11435 15775 17159 17186 17764 17793 17794 17795 17796 18127 18128 18129 18131 19009 19309 19393 25218 25289 25429 26508 26516
PDB
DBJ BAA22264 BAA84580 BAB71958 BAD51938 BAE01907
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GB AAA35540 AAA36829 AAA51564 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014
SP P05067 P53601 P79307 P86906 Q28053
TPG DAA33655