BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17914

Title: Integral membrane core domain of the mercury transporter MerF in lipid bilayer membranes   PubMed: 22217388

Authors: Das, Bibhuti; Nothnagel, Henry; Lu, George; Son, Woo Sung; Park, Sang Ho; Tian, Ye; Marassi, Francesca; Opella, Stanley

Citation: Das, Bibhuti; Nothnagel, Henry; Lu, George; Son, Woo Sung; Tian, Ye; Marassi, Francesca; Opella, Stanley. "Structure determination of a membrane protein in proteoliposomes."  J. Am. Chem. Soc. 134, 2047-2056 (2012).

Assembly members:
MerFt, polymer, 60 residues, 6281.516 Da.
ANI, non-polymer, 161.124 Da.

Natural source:   Common Name: Morganella morganii   Taxonomy ID: 582   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Morganella morganii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MerFt: IGTTLVALSSFTPVLVILLG VVGLSALTGYLDYVLLPALA IFIGLTIYAIQRKRQADASS

Data sets:
Data typeCount
13C chemical shifts110
15N chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MerFt1
2ANI2

Entities:

Entity 1, MerFt 60 residues - 6281.516 Da.

1   ILEGLYTHRTHRLEUVALALALEUSERSER
2   PHETHRPROVALLEUVALILELEULEUGLY
3   VALVALGLYLEUSERALALEUTHRGLYTYR
4   LEUASPTYRVALLEULEUPROALALEUALA
5   ILEPHEILEGLYLEUTHRILETYRALAILE
6   GLNARGLYSARGGLNALAASPALASERSER

Entity 2, ANI - C7 H6 F3 N - 161.124 Da.

1   ANI

Samples:

sample_1: MerFt, [U-100% 13C; U-100% 15N], 3.6 mg; H2O 100.0%

sample_conditions_1: ionic strength: 10 mM; pH: 6.1; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
see publicationsample_1anisotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

BMRB 19115
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