BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17774

Title: Antiparallel beta-sheet structural model for Iowa-mutant beta-amyloid fibrils   PubMed: 22403062

Authors: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert

Citation: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert. "Antiparallel -sheet architecture in Iowa-mutant -amyloid fibrils."  Proc. Natl. Acad. Sci. U.S.A. 109, 4443-4448 (2012).

Assembly members:
entity, polymer, 26 residues, 2650.167 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: QKLVFFAENVGSNKGAIIGL MVGGVV

Data sets:
Data typeCount
13C chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abeta1

Entities:

Entity 1, Abeta 26 residues - 2650.167 Da.

1   GLNLYSLEUVALPHEPHEALAGLUASNVAL
2   GLYSERASNLYSGLYALAILEILEGLYLEU
3   METVALGLYGLYVALVAL

Samples:

sample_1: Abeta, [U-13C], 25 uM; H2O 100%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C fpRFDRsample_1solidsample_conditions_1
2D 13C-13C RADsample_1solidsample_conditions_1
2D 13C-13C CHHCsample_1solidsample_conditions_1
1D 13C PITHIRDs-CTsample_1solidsample_conditions_1

Software:

X-PLOR NIH v2.28.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Varian InfinityPlus 400 MHz

Related Database Links:

BMRB 18175 25004
PDB