BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17761

Title: Backbone 1H, 13C, 15N and 13CB assignment of N-terminal Domain of Ribosomal Protein L9 (NTL9) Denatured State in 8.3M Urea   PubMed: 21915914

Authors: Meng, Wenli; Raleigh, Daniel

Citation: Meng, Wenli; Raleigh, Daniel. "Analysis of electrostatic interactions in the denatured state ensemble of the N-terminal domain of L9 under native conditions."  Proteins 79, 3500-3510 (2011).

Assembly members:
N-terminal Domain of the Ribosomal Protein L9, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus stearothermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-terminal Domain of the Ribosomal Protein L9: MKVIFLKDVKGKGKKGEIKN VADGYANNFLFKQGLAIEAT PANLKALEAQKQKEQR

Data sets:
Data typeCount
13C chemical shifts161
15N chemical shifts54
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal Domain of the Ribosomal Protein L91

Entities:

Entity 1, N-terminal Domain of the Ribosomal Protein L9 56 residues - Formula weight is not available

1   METLYSVALILEPHELEULYSASPVALLYS
2   GLYLYSGLYLYSLYSGLYGLUILELYSASN
3   VALALAASPGLYTYRALAASNASNPHELEU
4   PHELYSGLNGLYLEUALAILEGLUALATHR
5   PROALAASNLEULYSALALEUGLUALAGLN
6   LYSGLNLYSGLUGLNARG

Samples:

sample_1: sodium chloride 100 mM; sodium acetate 20 mM; urea 8.3 M; DSS 0.4 mM; N-terminal Domain of the Ribosomal Protein L9, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_2: sodium chloride 100 mM; sodium acetate 20 mM; urea 8.3 M; DSS 0.4 mM; N-terminal Domain of the Ribosomal Protein L9, [U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.12 M; pH: 5.5; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 17460 17506 4551
PDB
EMBL CAA43972
GB AAA22701 ABO68755 AGT33858 AKM20686 AKU26730
PRF 0701226A 1714237A
REF WP_008880809 WP_011888453 WP_020961638 WP_033008865 WP_050368025
SP A4ITV1 P02417