BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17728

Title: 13C and 15N Chemical Shift Assignments for the fd Bacteriophage   PubMed: 21702439

Authors: Goldbourt, Amir; Abramov, Gili; Morag, Omry

Citation: Abramov, Gili; Morag, Omry; Goldbourt, Amir. "Magic-angle spinning NMR of a class I filamentous bacteriophage virus"  J. Phys. Chem. B. 115, 9671-9680 (2011).

Assembly members:
fd, polymer, 50 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli

Entity Sequences (FASTA):
fd: AEGDDPAKAAFDSLQASATE YIGYAWAMVVVIVGATIGIK LFKKFTSKAS

Data sets:
Data typeCount
13C chemical shifts206
15N chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1coat protein1

Entities:

Entity 1, coat protein 50 residues - Formula weight is not available

1   ALAGLUGLYASPASPPROALALYSALAALA
2   PHEASPSERLEUGLNALASERALATHRGLU
3   TYRILEGLYTYRALATRPALAMETVALVAL
4   VALILEVALGLYALATHRILEGLYILELYS
5   LEUPHELYSLYSPHETHRSERLYSALASER

Samples:

sample_1: fd, [U-99% 13C; U-99% 15N], 7 – 15 mg; Tris

sample_conditions_1: ionic strength: 10 mM; pH: 8; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D 13C-13C RFDRsample_1solidsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19734 19747 2590 4197 4209
PDB
DBJ BAF74207 BAF74217
EMBL CAA23861 CAA23871
GB AAA32207 AAA32214 AAA32220 AAA32308 AAB24445
PRF 0812197K 690935A
REF NP_510890 YP_009111292 YP_009111302
SP P03618 P69539 P69540 P69541