BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17708

Title: Solution-state structures of monomeric and dimeric G-quadruplexes adopted by a sequence from N-myc   PubMed: 22303871

Authors: Trajkovski, Marko; Plavec, Janez; Webba da Silva, Mateus

Citation: Trajkovski, Marko; Webba da Silva, Mateus; Plavec, Janez. "Unique Structural Features of Interconverting Monomeric and Dimeric G-Quadruplexes Adopted by a Sequence from the Intron of the N-myc Gene."  J. Am. Chem. Soc. 134, 4132-4141 (2012).

Assembly members:
N-myc, polymer, 19 residues, 6064.968 Da.

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
N-myc: TAGGGCGGGAGGGAGGGAA

Data sets:
Data typeCount
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-myc, chain a1
2N-myc, chain b1

Entities:

Entity 1, N-myc, chain a 19 residues - 6064.968 Da.

1   DTDADGDGDGDCDGDGDGDA
2   DGDGDGDADGDGDGDADA

Samples:

sample_1: N-myc0.4 – 6.6 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
JRHMBCsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - structure solution

NMR spectrometers:

  • Varian VNMRS 800 MHz
  • Varian VNMRS 600 MHz

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