BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17700

Title: 13C, 15N Chemical shifts of E. coli thioredoxin using solid-state nmr spectroscopy   PubMed: 16853329

Authors: Paramasivam, Sivakumar

Citation: Marulanda, Dabeiba; Tasayco, Maria Luisa; Cataldi, Marcela; Arriaran, Vilma; Polenova, Tatyana. "Resonance Assignments and Secondary Structure Analysis of E. coli Thioredoxin by Magic Angle Spinning Solid-State NMR Spectroscopy"  J. Phys. Chem. B 109, 18135-18145 (2005).

Assembly members:
TRX_intact, polymer, 108 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TRX_intact: SDKIIHLTDDSFDTDVLKAD GAILVDFWAEWCGPCKMIAP ILDEIADEYQGKLTVAKLNI DQNPGTAPKYGIRGIPTLLL FKNGEVAATKVGALSKGQLK EFLDANLA

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRX Intact1

Entities:

Entity 1, TRX Intact 108 residues - Formula weight is not available

1   SERASPLYSILEILEHISLEUTHRASPASP
2   SERPHEASPTHRASPVALLEULYSALAASP
3   GLYALAILELEUVALASPPHETRPALAGLU
4   TRPCYSGLYPROCYSLYSMETILEALAPRO
5   ILELEUASPGLUILEALAASPGLUTYRGLN
6   GLYLYSLEUTHRVALALALYSLEUASNILE
7   ASPGLNASNPROGLYTHRALAPROLYSTYR
8   GLYILEARGGLYILEPROTHRLEULEULEU
9   PHELYSASNGLYGLUVALALAALATHRLYS
10   VALGLYALALEUSERLYSGLYGLNLEULYS
11   GLUPHELEUASPALAASNLEUALA

Samples:

sample_1: TRX Intact, [U-100% 13C; U-100% 15N], mM; H2O 100%

sample_conditions_1: ionic strength: . M; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D C-C DARRsample_1solidsample_conditions_1
2D N-CA SPECIFIC CPsample_1solidsample_conditions_1
2D N-CO SPECIFIC CPsample_1solidsample_conditions_1
2D N-CA-CXsample_1solidsample_conditions_1
2D N-CO-CXsample_1solidsample_conditions_1

Software:

NMRPipe_and_SPARKY, Delaglio, Zhengrong and Bax, Goddard - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

BMRB 1812 1813
PDB
DBJ BAA00903 BAB38137 BAE77517 BAG79587 BAH61053
EMBL CAA79851 CAD09400 CAP78228 CAQ34125 CAQ91183
GB AAA24533 AAA24534 AAA24693 AAA24694 AAA24696
PIR AF0922 B91218 C86064
REF NP_312741 NP_418228 NP_457831 NP_462806 NP_709584
SP P0AA25 P0AA26 P0AA27 P0AA28 P0AA29