BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17692

Title: backbone resonance assignment of the DMSO-d6 denatured DLC8   PubMed: 22079190

Authors: Chakraborty, Swagata

Citation: Chakraborty, Swagata; Mohan, P. M Krishna; Hosur, Ramakrishna. "Residual structure and dynamics in DMSO-d6 denatured Dynein Light Chain protein."  Biochimie 94, 231-241 (2012).

Assembly members:
DLC8, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DLC8: MSDRKAVIKNADMSEEMQQD AVDCATQALEKYNIEKDIAA YIKKEFDKKYNPTWHCIVGR NFGSYVTHETRHFIYFYLGQ VAILLFKSG

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts88
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DLC81

Entities:

Entity 1, DLC8 89 residues - Formula weight is not available

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Samples:

sample_1: DLC8, [U-100% 13C; U-100% 15N], 1.2 – 1.5 mM; TRIS 20 mM; sodium chloride 200 mM; DTT 2 mM; DMSO 99.96%

sample_conditions_1: ionic strength: 0.2 M; pH: 2.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA, Rochus Keller - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15076 15077 15078 15953 16847
PDB
EMBL CDQ62741 CDQ75050
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF NP_001135068 NP_001177683 NP_001188181 NP_001245530 NP_001245836
SP O96860 Q24117
TPG DAA34424