BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17513

Title: Towards an atomistic level understanding of the unfolded state of Immunity protein 7 in non-denaturing conditions   PubMed: 22226836

Authors: Pashley, Clare; Morgan, Gareth; Kalverda, Arnout; Thompson, Gary; Radford, Sheena

Citation: Pashley, Clare; Morgan, Gareth; Kalverda, Arnout; Thompson, Gary; Kleanthous, Colin; Radford, Sheena. "Conformational properties of the unfolded state of Im7 in nondenaturing conditions."  J. Mol. Biol. 416, 300-318 (2012).

Assembly members:
Immunity protein 7, polymer, 94 residues, 10717.6621 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Immunity protein 7: MEHHHHHHELKNSISDYTEA EFVQAAKEIEKENVAATDDV LDVALEHFVKITEHPDGTDL IYYPSDNRDDSPEGIVKEIK EWRAANGKPGFKQG

Data sets:
Data typeCount
13C chemical shifts472
15N chemical shifts162
1H chemical shifts231
coupling constants58
heteronuclear NOE values62
T2 relaxation values60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Immunity protein 71

Entities:

Entity 1, Immunity protein 7 94 residues - 10717.6621 Da.

1   METGLUHISHISHISHISHISHISGLULEU
2   LYSASNSERILESERASPTYRTHRGLUALA
3   GLUPHEVALGLNALAALALYSGLUILEGLU
4   LYSGLUASNVALALAALATHRASPASPVAL
5   LEUASPVALALALEUGLUHISPHEVALLYS
6   ILETHRGLUHISPROASPGLYTHRASPLEU
7   ILETYRTYRPROSERASPASNARGASPASP
8   SERPROGLUGLYILEVALLYSGLUILELYS
9   GLUTRPARGALAALAASNGLYLYSPROGLY
10   PHELYSGLNGLY

Samples:

0.5mM: Molecule 1, [U-13C; U-15N], 0.5 mM; sodium phosphate 50 mM; sodium sulfate 0.2 M; EDTA 1 mM; H2O 90%; D2O 10%

urea: Molecule 1, [U-13C; U-15N], 0.5 mM; urea 6 M; sodium phosphate 50 mM; sodium sulfate 0.2 M; EDTA 1 mM; H2O 90%; D2O 10%

Normal: ionic strength: 0.610 M; pH: 7.000; pressure: 1.000 atm; temperature: 283.000 K

sample_conditions_urea: ionic strength: 0.610 M; pH: 7.000; pressure: 1.000 atm; temperature: 283.000 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NH0.5mMisotropicNormal
2D 1H-15N HSQC/HMQC0.5mMisotropicNormal
3D HNCA0.5mMisotropicNormal
HNCACO (H[N[ca[CO]]])0.5mMisotropicNormal
3D HNCO0.5mMisotropicNormal
3D HNCACB0.5mMisotropicNormal
Expt_10 (h[N]_H[N].NOESY)0.5mMisotropicNormal
Expt_12 (H{[N]+[HA]})0.5mMisotropicNormal
Expt_13 (h[N]_H.NOESY)0.5mMisotropicNormal
2D 1H-15N HSQCureaisotropicsample_conditions_urea
3D HNCAureaisotropicsample_conditions_urea
3D HNCOureaisotropicsample_conditions_urea
3D HNCACOureaisotropicsample_conditions_urea
3D CBCA(CO)NHureaisotropicsample_conditions_urea

Software:

ANALYSIS v1.0, CCPN - Spectrum analysis, Spectrum display

NMRPipe vany, Frank Delagio, Stephan Grzesiek, Guang Zhu, Geerten W. Vuister, John Pfiefer and Ad Bax - spectrum analysis, Spectrum processing

NMR spectrometers:

  • Varian UnityInova 500 MHz
  • Varian UnityInova 749 MHz

Related Database Links:

BMRB TmpAcc