BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17499

Title: Solid-state NMR assignment of the Ure2p prion C-terminal 70-354 in microcrystalline form.   PubMed: 21805376

Authors: Habenstein, Birgit; Wasmer, Christian; Bousset, Luc; Sourigues, Yannick; Schuetz, Anne; Loquet, Antoine; Meier, Beat; Melki, Ronald; Bockmann, Anja

Citation: Habenstein, Birgit; Wasmer, Christian; Bousset, Luc; Sourigues, Yannick; Schutz, Anne; Loquet, Antoine; Meier, Beat; Melki, Ronald; Bockmann, Anja. "Extensive de novo solid-state NMR assignments of the 33 kDa C-terminal domain of the Ure2 prion."  J. Biomol. NMR 51, 235-243 (2011).

Assembly members:
Ure2p70-354, polymer, 295 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ure2p70-354: MNNDNENNIKNTLEQHRQQQ QAFSDMSHVEYSRITKFFQE QPLEGYTLFSHRSAPNGFKV AIVLSELGFHYNTIFLDFNL GEHRAPEFVSVNPNARVPAL IDHGMDNLSIWESGAILLHL VNKYYKETGNPLLWSDDLAD QSQINAWLFFQTSGHAPMIG QALHFRYFHSQKIASAVERY TDEVRRVYGVVEMALAERRE ALVMELDTENAAAYSAGTTP MSQSRFFDYPVWLVGDKLTI ADLAFVPWNNVVDRIGINIK IEFPEVYKWTKHMMRRPAVI KALRGERGSHHHHHH

Data sets:
Data typeCount
13C chemical shifts751
15N chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ure2p70-3541

Entities:

Entity 1, Ure2p70-354 295 residues - Formula weight is not available

RGSHHHHHH at the C-terminus is the non-native His-tag

1   METASNASNASPASNGLUASNASNILELYS
2   ASNTHRLEUGLUGLNHISARGGLNGLNGLN
3   GLNALAPHESERASPMETSERHISVALGLU
4   TYRSERARGILETHRLYSPHEPHEGLNGLU
5   GLNPROLEUGLUGLYTYRTHRLEUPHESER
6   HISARGSERALAPROASNGLYPHELYSVAL
7   ALAILEVALLEUSERGLULEUGLYPHEHIS
8   TYRASNTHRILEPHELEUASPPHEASNLEU
9   GLYGLUHISARGALAPROGLUPHEVALSER
10   VALASNPROASNALAARGVALPROALALEU
11   ILEASPHISGLYMETASPASNLEUSERILE
12   TRPGLUSERGLYALAILELEULEUHISLEU
13   VALASNLYSTYRTYRLYSGLUTHRGLYASN
14   PROLEULEUTRPSERASPASPLEUALAASP
15   GLNSERGLNILEASNALATRPLEUPHEPHE
16   GLNTHRSERGLYHISALAPROMETILEGLY
17   GLNALALEUHISPHEARGTYRPHEHISSER
18   GLNLYSILEALASERALAVALGLUARGTYR
19   THRASPGLUVALARGARGVALTYRGLYVAL
20   VALGLUMETALALEUALAGLUARGARGGLU
21   ALALEUVALMETGLULEUASPTHRGLUASN
22   ALAALAALATYRSERALAGLYTHRTHRPRO
23   METSERGLNSERARGPHEPHEASPTYRPRO
24   VALTRPLEUVALGLYASPLYSLEUTHRILE
25   ALAASPLEUALAPHEVALPROTRPASNASN
26   VALVALASPARGILEGLYILEASNILELYS
27   ILEGLUPHEPROGLUVALTYRLYSTRPTHR
28   LYSHISMETMETARGARGPROALAVALILE
29   LYSALALEUARGGLYGLUARGGLYSERHIS
30   HISHISHISHISHIS

Samples:

sample_1: Ure2p70-354, [U-100% 13C; U-100% 15N], 20 – 40 mg; KCl 75 mM; TRIS 20 mM; EGTA 1 mM; dithiothreitol 1 mM; DSS 0.5 mg; H2O 100%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D NCACB (DREAM)sample_1solidsample_conditions_1
3D N(CO)CACBsample_1solidsample_conditions_1
3D CAN(CO)CAsample_1solidsample_conditions_1
3D CCCsample_1solidsample_conditions_1
3D NCACX (DARR)sample_1solidsample_conditions_1

Software:

ANALYSIS v2.1.1, CCPN - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

BMRB 17570
PDB
DBJ BAM78703 GAA25878
EMBL CAA93369 CAA96134 CAY82378
GB AAA35201 AAK51641 AAM91938 AAM91939 AAM93167
REF NP_014170
SP P23202 Q7LLZ8 Q8NJR6
TPG DAA10329