BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17367

Title: Assignments of Drosophila cytotoxic T-lymphocyte antigen 2 (CTLA-2) like protein, crammer at pH3   PubMed: 22150223

Authors: Tzeng, Tien-Sheng; Hsu, Shang-Te Danny; Lyu, Ping-Chiang

Citation: Tseng, Tien-Sheng; Cheng, Chao-Sheng; Chen, Dian-Jiun; Shih, Min-Fang; Liu, Yu-Nan; Hsu, Shang-Te; Lyu, Ping-Chiang. "A Molten Globule-to-Ordered Structure Transition of Drosophila melanogaster Crammer Is Required for its Ability to Inhibit Cathepsin"  Biochem. J. 442, 563-572 (2012).

Assembly members:
C72S_monomer, polymer, 80 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
C72S_monomer: MMSLVSDEEWVEYKSKFDKN YEAEEDLMRRRIYAESKARI EEHNRKFEKGEVTWKMGINH LADLTPEEFAQRSGKKVPPN

Data sets:
Data typeCount
13C chemical shifts224
15N chemical shifts74
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C72S monomer1

Entities:

Entity 1, C72S monomer 80 residues - Formula weight is not available

1   METMETSERLEUVALSERASPGLUGLUTRP
2   VALGLUTYRLYSSERLYSPHEASPLYSASN
3   TYRGLUALAGLUGLUASPLEUMETARGARG
4   ARGILETYRALAGLUSERLYSALAARGILE
5   GLUGLUHISASNARGLYSPHEGLULYSGLY
6   GLUVALTHRTRPLYSMETGLYILEASNHIS
7   LEUALAASPLEUTHRPROGLUGLUPHEALA
8   GLNARGSERGLYLYSLYSVALPROPROASN

Samples:

sample_1: C72S_monomer, [U-98% 13C; U-98% 15N], 0.5 mM

sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY_and_NMRpipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, processing

NMR spectrometers:

  • Varian INOVA 700 MHz

Related Database Links:

BMRB 16719
PDB
GB AAF57567 ACJ13252 EDV55205 EDW48595 EDX07811
REF NP_611420 XP_001974805 XP_002034582 XP_002082226