BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17361

Title: The Conformation of Bacteriorhodopsin Loops in Purple Membranes resolved by Solid-state MAS-NMR   PubMed: 21770003

Authors: Higman, Victoria; Varga, Krisztina; Aslimovska, Lubica; Judge, Peter; Sperling, Lindsay; Rienstra, Chad; Watts, Anthony

Citation: Higman, Victoria; Varga, Krisztina; Aslimovska, Lubica; Judge, Peter; Sperling, Lindsay; Rienstra, Chad; Watts, Anthony. "The conformation of bacteriorhodopsin loops in purple membranes resolved by solid-state MAS NMR spectroscopy."  Angew. Chem. Int. Ed. Engl. 50, 8432-8435 (2011).

Assembly members:
bR, polymer, 249 residues, Formula weight is not available

Natural source:   Common Name: Halobacterium salinarum   Taxonomy ID: 2242   Superkingdom: Archaea   Kingdom: not available   Genus/species: Halobacterium salinarum

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
bR: QAQITGRPEWIWLALGTALM GLGTLYFLVKGMGVSDPDAK KFYAITTLVPAIAFTMYLSM LLGYGLTMVPFGGEQNPIYW ARYADWLFTTPLLLLDLALL VDADQGTILALVGADGIMIG TGLVGALTKVYSYRFVWWAI STAAMLYILYVLFFGFTSKA ESMRPEVASTFKVLRNVTVV LWSAYPVVWLIGSEGAGIVP LNIETLLFMVLDVSAKVGFG LILLRSRAIFGEAEAPEPSA GDGAAATSD

Data sets:
Data typeCount
13C chemical shifts204
15N chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bR1

Entities:

Entity 1, bR 249 residues - Formula weight is not available

1   GLNALAGLNILETHRGLYARGPROGLUTRP
2   ILETRPLEUALALEUGLYTHRALALEUMET
3   GLYLEUGLYTHRLEUTYRPHELEUVALLYS
4   GLYMETGLYVALSERASPPROASPALALYS
5   LYSPHETYRALAILETHRTHRLEUVALPRO
6   ALAILEALAPHETHRMETTYRLEUSERMET
7   LEULEUGLYTYRGLYLEUTHRMETVALPRO
8   PHEGLYGLYGLUGLNASNPROILETYRTRP
9   ALAARGTYRALAASPTRPLEUPHETHRTHR
10   PROLEULEULEULEUASPLEUALALEULEU
11   VALASPALAASPGLNGLYTHRILELEUALA
12   LEUVALGLYALAASPGLYILEMETILEGLY
13   THRGLYLEUVALGLYALALEUTHRLYSVAL
14   TYRSERTYRARGPHEVALTRPTRPALAILE
15   SERTHRALAALAMETLEUTYRILELEUTYR
16   VALLEUPHEPHEGLYPHETHRSERLYSALA
17   GLUSERMETARGPROGLUVALALASERTHR
18   PHELYSVALLEUARGASNVALTHRVALVAL
19   LEUTRPSERALATYRPROVALVALTRPLEU
20   ILEGLYSERGLUGLYALAGLYILEVALPRO
21   LEUASNILEGLUTHRLEULEUPHEMETVAL
22   LEUASPVALSERALALYSVALGLYPHEGLY
23   LEUILELEULEUARGSERARGALAILEPHE
24   GLYGLUALAGLUALAPROGLUPROSERALA
25   GLYASPGLYALAALAALATHRSERASP

Samples:

sample_1: bR, [U-100% 13C; U-100% 15N], 18 mg; sodium citrate 20 mM; sodium azide 0.01%; H2O 95%; D2O 5%; purple membrane lipids, [U-100% 13C; U-100% 15N], 6 mg

sample_conditions_1: ionic strength: 0.2 mM; pH: 6.0; pressure: 1 atm; temperature: 278 K

sample_conditions_2: ionic strength: 0.2 mM; pH: 6.0; pressure: 1 atm; temperature: 263 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_1solidsample_conditions_1
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_2
CANCOCXsample_1isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis v2.1.15, CCPN - chemical shift assignment, peak picking

Spinsight, Chemagnetics/Varian - collection

NMR spectrometers:

  • Varian Infinity Plus 800 MHz
  • Varian Infinity Plus 500 MHz

Related Database Links:

PDB
EMBL CAA23744 CAA49762 CAA49774 CAP14056
GB AAA72184 AAA72504 AAA72603 AAG19772
PRF 0501217A 0712242A
REF WP_049892510
SP P02945