BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17064

Title: 13C, and 15N Chemical Shift Assignments for Anabaena sensory rhodopsin 1-229   PubMed: 21191921

Authors: Shi, Lichi; Kawamura, Izuru; Jung, Kwang-Hwang; Brown, Leonid; Ladizhansky, Vladimir

Citation: Shi, Lichi; Kawamura, Izuru; Jung, Kwang-Hwan; Brown, Leonid; Ladizhansky, Vladimir. "Conformation of a Seven-Helical Transmembrane Photosensor in the Lipid Environment."  Angew. Chem. Int. Ed. Engl. ., .-. (2010).

Assembly members:
opsin, polymer, 235 residues, 27220 Da.
RET, non-polymer, 284.436 Da.

Natural source:   Common Name: Anabaena PCC7120   Taxonomy ID: 103690   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
opsin: MNLESLLHWIYVAGMTIGAL HFWSLSRNPRGVPQYEYLVA MFIPIWSGLAYMAMAIDQGK VEAAGQIAHYARYIDWMVTT PLLLLSLSWTAMQFIKKDWT LIGFLMSTQIVVITSGLIAD LSERDWVRYLWYICGVCAFL IILWGIWNPLRAKTRTQSSE LANLYDKLVTYFTVLWIGYP IVWIIGPSGFGWINQTIDTF LFCLLPFFSKVGFSFLDLHG LRNLNDSRQHHHHHH

Data sets:
Data typeCount
13C chemical shifts668
15N chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1opsin1
2retinal2

Entities:

Entity 1, opsin 235 residues - 27220 Da.

1   METASNLEUGLUSERLEULEUHISTRPILE
2   TYRVALALAGLYMETTHRILEGLYALALEU
3   HISPHETRPSERLEUSERARGASNPROARG
4   GLYVALPROGLNTYRGLUTYRLEUVALALA
5   METPHEILEPROILETRPSERGLYLEUALA
6   TYRMETALAMETALAILEASPGLNGLYLYS
7   VALGLUALAALAGLYGLNILEALAHISTYR
8   ALAARGTYRILEASPTRPMETVALTHRTHR
9   PROLEULEULEULEUSERLEUSERTRPTHR
10   ALAMETGLNPHEILELYSLYSASPTRPTHR
11   LEUILEGLYPHELEUMETSERTHRGLNILE
12   VALVALILETHRSERGLYLEUILEALAASP
13   LEUSERGLUARGASPTRPVALARGTYRLEU
14   TRPTYRILECYSGLYVALCYSALAPHELEU
15   ILEILELEUTRPGLYILETRPASNPROLEU
16   ARGALALYSTHRARGTHRGLNSERSERGLU
17   LEUALAASNLEUTYRASPLYSLEUVALTHR
18   TYRPHETHRVALLEUTRPILEGLYTYRPRO
19   ILEVALTRPILEILEGLYPROSERGLYPHE
20   GLYTRPILEASNGLNTHRILEASPTHRPHE
21   LEUPHECYSLEULEUPROPHEPHESERLYS
22   VALGLYPHESERPHELEUASPLEUHISGLY
23   LEUARGASNLEUASNASPSERARGGLNHIS
24   HISHISHISHISHIS

Entity 2, retinal - C20 H28 O - 284.436 Da.

1   RET

Samples:

sample_1: opsin, [U-99% 13C; U-99% 15N], 11 ± 1 mg; retinal 110 ± 10 ug; H2O 100%; NaCl 10 mM; CHES 25 mM

sample_conditions_1: ionic strength: 90 mM; pH: 9; pressure: 1 atm; temperature: 273 K

sample_conditions_2: ionic strength: 90 mM; pD: 9; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D CONCAsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18595 25290
PDB
DBJ BAB74864
REF WP_010997316