BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16501

Title: (1-153)Apomyoglobin   PubMed: 20816043

Authors: Fedyukina, Daria; Cavagnero, Silvia

Citation: Fedyukina, Daria; Rajagopalan, Senapathy; Sekhar, Ashok; Fulmer, Eric; Eun, Ye-Jin; Cavagnero, Silvia. "Contribution of long-range interactions to the secondary structure of an unfolded globin."  Biophys. J. 99, L37-L39 (2010).

Assembly members:
(1-153)Apomyoglobin, polymer, 154 residues, Formula weight is not available

Natural source:   Common Name: Sperm Whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
(1-153)Apomyoglobin: MVLSEGEWQLVLHVWAKVEA DVAGHGQDILIRLFKSHPET LEKFDRFKHLKTEAEMKASE DLKKHGVTVLTALGAILKKK GHHEAELKPLAQSHATKHKI PIKYLEFISEAIIHVLHSRH PGDFGADAQGAMNKALELFR KDIAAKYKELGYQG

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts141
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(1-153)Apomyoglobin1

Entities:

Entity 1, (1-153)Apomyoglobin 154 residues - Formula weight is not available

1   METVALLEUSERGLUGLYGLUTRPGLNLEU
2   VALLEUHISVALTRPALALYSVALGLUALA
3   ASPVALALAGLYHISGLYGLNASPILELEU
4   ILEARGLEUPHELYSSERHISPROGLUTHR
5   LEUGLULYSPHEASPARGPHELYSHISLEU
6   LYSTHRGLUALAGLUMETLYSALASERGLU
7   ASPLEULYSLYSHISGLYVALTHRVALLEU
8   THRALALEUGLYALAILELEULYSLYSLYS
9   GLYHISHISGLUALAGLULEULYSPROLEU
10   ALAGLNSERHISALATHRLYSHISLYSILE
11   PROILELYSTYRLEUGLUPHEILESERGLU
12   ALAILEILEHISVALLEUHISSERARGHIS
13   PROGLYASPPHEGLYALAASPALAGLNGLY
14   ALAMETASNLYSALALEUGLULEUPHEARG
15   LYSASPILEALAALALYSTYRLYSGLULEU
16   GLYTYRGLNGLY

Samples:

sample_1: (1-153)Apomyoglobin, [U-99% 13C; U-99% 15N], 304 uM; CD3COOH 5 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 2.40; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676 4695
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185