BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16060

Title: Loop 3 of Short Neurotoxin II is an Additional Interaction Site with Membrane-Bound Nicotinic Acetylcholine Receptor as Detected by Solid-State NMR Spectroscopy   PubMed: 19447114

Authors: van Rossum, Barth-Jan; Oschkinat, Hartmut

Citation: Krabben, Ludwig; van Rossum, Barth-Jan; Jehle, Stefan; Bocharov, Eduard; Lyukmanova, Ekaterina; Schulga, Alexey; Arseniev, Alexander; Hucho, Ferdinand; Oschkinat, Hartmut. "Loop 3 of Short Neurotoxin II is an Additional Interaction Site with Membrane-Bound Nicotinic Acetylcholine Receptor as Detected by Solid-State NMR Spectroscopy"  J. Mol. Biol. 390, 662-671 (2009).

Assembly members:
NT_II, polymer, 61 residues, Formula weight is not available
nAChR, polymer, . residues, Formula weight is not available

Natural source:   Common Name: Central Asian cobra   Taxonomy ID: 8657   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Naja oxiana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NT_II: LECHNQQSSQPPTTKTCSGE TNCYKKWWSDHRGTIIERGC GCPKVKPGVNLNCCRTDRCN N
nAChR: X

Data sets:
Data typeCount
13C chemical shifts272
15N chemical shifts60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1neurotoxin II1
2nicotinic Acetylcholin receptor2

Entities:

Entity 1, neurotoxin II 61 residues - Formula weight is not available

1   LEUGLUCYSHISASNGLNGLNSERSERGLN
2   PROPROTHRTHRLYSTHRCYSSERGLYGLU
3   THRASNCYSTYRLYSLYSTRPTRPSERASP
4   HISARGGLYTHRILEILEGLUARGGLYCYS
5   GLYCYSPROLYSVALLYSPROGLYVALASN
6   LEUASNCYSCYSARGTHRASPARGCYSASN
7   ASN

Entity 2, nicotinic Acetylcholin receptor - Formula weight is not available

1   X

Samples:

neurotoxin: NT II, [U-100% 13C; U-100% 15N],

13neurotoxin: NT II, [1,3-13C]-Glycerol; [U-15N],

2neurotoxin: NT II, [2-13C]-Glycerol; [U-15N],

nAChR_sample: nAChR

NTII-nAChR_complex: temperature: 265 K

Experiments:

NameSampleSample stateSample conditions
2D PDSDneurotoxinsolidNTII-nAChR_complex
2D PDSD13neurotoxinsolidNTII-nAChR_complex
2D PDSD2neurotoxinsolidNTII-nAChR_complex
2D TEDOR13neurotoxinsolidNTII-nAChR_complex
2D TEDOR2neurotoxinsolidNTII-nAChR_complex

Software:

SPARKY v3.1, Goddard - chemical shift assignment

xwinnmr v2.6, Bruker Biospin - collection

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 400 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19704
PDB
SP P01427 P59276