BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16041

Title: FLNCCPGCCMEP_ReAsH_motif   PubMed: 19281235

Authors: Madani, Fatemeh; Lind, Jesper; Damberg, Peter; Adams, Stephen; Tsien, Roger; Graslund, Astrid

Citation: Madani, Fatemeh; Lind, Jesper; Damberg, Peter; Adams, Stephen; Tsien, Roger; Graslund, Astrid. "Hairpin Structure of a Biarsenical-Tetracysteine Motif Determined by NMR"  J. Am. Chem. Soc. 131, 4613-4615 (2009).

Assembly members:
FLNCCPGCCMEP, polymer, 12 residues, Formula weight is not available
ReAsH, non-polymer, 360 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
FLNCCPGCCMEP: FLNCCPGCCMEP

Data sets:
Data typeCount
1H chemical shifts66
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLNCCPGCCMEP1
2ReAsH, resorufin arsenical hairpin binder2

Entities:

Entity 1, FLNCCPGCCMEP 12 residues - Formula weight is not available

1   PHELEUASNCYSCYSPROGLYCYSCYSMET
2   GLUPRO

Entity 2, ReAsH, resorufin arsenical hairpin binder - C12 H4 N O3 As2 - 360 Da.

Samples:

sample_1: FLNCCPGCCMEP 0.6 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Varian INOVA 600 MHz

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