BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16020

Title: Magic-Angle-Spinning Solid-State NMR Structure of Influenza A M2 Transmembrane Domain   PubMed: 19061899

Authors: Hong, Mei; Cady, Sarah; Mishanina, Tatiana

Citation: Cady, Sarah; Mishanina, Tatiana; Hong, Mei. "Structure of Amantadine-Bound M2 Transmembrane Peptide of Influenza A in Lipid Bilayers from Magic-Angle-Spinning Solid-State NMR: the Role of Ser31 in Amantadine Binding"  J. Mol. Biol. 385, 1127-1141 (2009).

Assembly members:
M2_Transmembrane_Peptide, polymer, 25 residues, 2688.241 Da.
308, non-polymer, 151.249 Da.

Natural source:   Common Name: Influenzavirus A   Taxonomy ID: 197911   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenzavirus A not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
M2_Transmembrane_Peptide: SSDPLVVAASIIGILHLIAW ILDRL

Data sets:
Data typeCount
13C chemical shifts51
15N chemical shifts11

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M2 Transmembrane Peptide_11
2M2 Transmembrane Peptide_21
3M2 Transmembrane Peptide_31
4M2 Transmembrane Peptide_41
5AMANTADINE2

Entities:

Entity 1, M2 Transmembrane Peptide_1 25 residues - 2688.241 Da.

1   SERSERASPPROLEUVALVALALAALASER
2   ILEILEGLYILELEUHISLEUILEALATRP
3   ILELEUASPARGLEU

Entity 2, AMANTADINE - C10 H17 N - 151.249 Da.

1   308

Samples:

M2_Transmembrane_Peptide_sample: M2 Transmembrane Peptide, Selective U-13C, 15N labeled, 4 – 6 mg; Amantadine15 – 25 mg; DLPC15 – 25 mg; sodium phosphate 10 mM; sodium azide .1 mM; EDTA 1 mM; H2O20 – 25 mg

M2_Transmembrane_Peptide_conditions: pH: 7.5; pressure: 1 atm; temperature: 243 K

Experiments:

NameSampleSample stateSample conditions
2D_13C-13C_DQF_COSYM2_Transmembrane_Peptide_samplesolidM2_Transmembrane_Peptide_conditions
2D_15N-13C_HETCORM2_Transmembrane_Peptide_samplesolidM2_Transmembrane_Peptide_conditions
2D_DARRM2_Transmembrane_Peptide_samplesolidM2_Transmembrane_Peptide_conditions
2D_INADEQUATEM2_Transmembrane_Peptide_samplesolidM2_Transmembrane_Peptide_conditions

Software:

TOPSPIN v1.3, Bruker Biospin - collection

InsightII v2005, Accelrys Software Inc. - structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker DRX 400 MHz

Related Database Links:

PDB
GB AAZ16348