BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15936

Title: Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30%   PubMed: 19847776

Authors: Kaczka, Piotr; Polkowska, Agnieszka

Citation: Kaczka, Piotr; Polkowska-Nowakowska, Agnieszka; Bolewska, Krystyna; Zhukov, Igor; Poznaski, Jarosaw; Wierzchowski, Kazimierz. "Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit."  Proteins 78, 754-768 (2010).

Assembly members:
4_sigma70, polymer, 107 residues, 12032.5 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
4_sigma70: MGSSHHHHHHSSGLVPRGSH MLELPLDSATTESLRAATHD VLAGLTAREAKVLRMRFGID MNTDYTLEEVGKQFDVTRER IRQIEAKALRKLRHPSRSEV LRSFLDD

Data typeCount
15N chemical shifts1000
1H chemical shifts1000

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14 sigma701

Entities:

Entity 1, 4 sigma70 107 residues - 12032.5 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METLEUGLULEUPROLEUASPSERALATHR
4   THRGLUSERLEUARGALAALATHRHISASP
5   VALLEUALAGLYLEUTHRALAARGGLUALA
6   LYSVALLEUARGMETARGPHEGLYILEASP
7   METASNTHRASPTYRTHRLEUGLUGLUVAL
8   GLYLYSGLNPHEASPVALTHRARGGLUARG
9   ILEARGGLNILEGLUALALYSALALEUARG
10   LYSLEUARGHISPROSERARGSERGLUVAL
11   LEUARGSERPHELEUASPASP

Samples:

sample_1: 4 sigma70, [U-13C; U-15N], 3 mM

sample_2: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 0.3%

sample_3: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 2%

sample_4: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 4%

sample_5: 4 of sigma70, [U-13C; U-15N], 3 mM; TFE 6%

sample_6: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 10%

sample_7: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 12%

sample_8: 4 sigma70 3 mM; TFE 15%

sample_9: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 20%

sample_10: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 30%

sample_conditions_all: ionic strength: 0.0 M; pH: 4.55; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_all
2D 1H-15N HSQCsample_2isotropicsample_conditions_all
2D 1H-15N HSQCsample_3isotropicsample_conditions_all
2D 1H-15N HSQCsample_4isotropicsample_conditions_all
2D 1H-15N HSQCsample_5isotropicsample_conditions_all
2D 1H-15N HSQCsample_6isotropicsample_conditions_all
2D 1H-15N HSQCsample_7isotropicsample_conditions_all
2D 1H-15N HSQCsample_8isotropicsample_conditions_all
2D 1H-15N HSQCsample_9isotropicsample_conditions_all
2D 1H-15N HSQCsample_10isotropicsample_conditions_all

Software:

NMRPipe v1.7, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.111, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 15930 15975
PDB
DBJ BAB37373 BAE77118 BAG66772 BAG78873 BAH65247
EMBL CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501
GB AAA24601 AAA27242 AAA89147 AAB60181 AAC76103
PIR AB0893
REF NP_311977 NP_417539 NP_457602 NP_462126 NP_708877
SP P00579 P0A2E3 P0A2E4