BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15918

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for M-crystallin (calcium bound form) in presence of 4 M Gdn-Hcl   PubMed: 19636958

Authors: Barnwal, Ravi Pratap; Agarwal, Geetika P; Chary, Kandala VR

Citation: Barnwal, Ravi; Agarwal, Geetika; Sharma, Yogendra; Chary, Kandala. "Complete backbone assignment of a Ca2+-binding protein of the betagamma-crystallin superfamily from Methanosarcina acetivorans, at two denaturant concentrations"  Biomol. NMR Assignments 3, 107-110 (2009).

Assembly members:
M-crystallin_(calcium_bound)_in_presence_of_4M_Gdn-HCl, polymer, 85 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
M-crystallin_(calcium_bound)_in_presence_of_4M_Gdn-HCl: MNAAEVIVYEHVNFGGKSFD ATSDQPGAGDNLNDKISSIK VKSGTWRFYEYINYGGRYWD LGPGEYSSVESAGIPDNSIS SFRQI

Data sets:
Data typeCount
13C chemical shifts208
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2CALCIUM ION2

Entities:

Entity 1, subunit 1 85 residues - Formula weight is not available

1   METASNALAALAGLUVALILEVALTYRGLU
2   HISVALASNPHEGLYGLYLYSSERPHEASP
3   ALATHRSERASPGLNPROGLYALAGLYASP
4   ASNLEUASNASPLYSILESERSERILELYS
5   VALLYSSERGLYTHRTRPARGPHETYRGLU
6   TYRILEASNTYRGLYGLYARGTYRTRPASP
7   LEUGLYPROGLYGLUTYRSERSERVALGLU
8   SERALAGLYILEPROASPASNSERILESER
9   SERPHEARGGLNILE

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: M-crystallin, [U-99% 13C; U-99% 15N], 1.2 mM; Ca2+ 6-10 mM; Gdn-Hcl 4000 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.8.3, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15934
PDB
GB AAM05909
REF WP_048065429