BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15883

Title: Backbone and 13C and 1H assignments for acid unfolded state and urea unfolded state of CTL9   PubMed: 18707127

Authors: Shan, Bing; Bhattacharya, Shibani; Eliezer, David; Raleigh, Daniel

Citation: Shan, Bing; Bhattacharya, Shibani; Eliezer, David; Raleigh, Daniel. "The Low pH Unfolded State of the C-terminal Domain of the Ribosomal Protein L9 Contains Significant Secondary Structure in the Absence of Denaturant but is No More Compact than the Low pH Urea Unfolded State"  Biochemistry 47, 9565-9573 (2008).

Assembly members:
CTL9, polymer, 92 residues, Formula weight is not available

Natural source:   Common Name: Bacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CTL9: AAEELANAKKLKEQLEKLTV TIPAKAGEGGRLFGSITSKQ IAESLQAQHGLKLDKRKIEL ADAIRALGYTNVPVKLHPEV TATLKVHVTEQK

Data sets:
Data typeCount
13C chemical shifts267
15N chemical shifts88
1H chemical shifts290

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CTL91

Entities:

Entity 1, CTL9 92 residues - Formula weight is not available

1   ALAALAGLUGLULEUALAASNALALYSLYS
2   LEULYSGLUGLNLEUGLULYSLEUTHRVAL
3   THRILEPROALALYSALAGLYGLUGLYGLY
4   ARGLEUPHEGLYSERILETHRSERLYSGLN
5   ILEALAGLUSERLEUGLNALAGLNHISGLY
6   LEULYSLEUASPLYSARGLYSILEGLULEU
7   ALAASPALAILEARGALALEUGLYTYRTHR
8   ASNVALPROVALLYSLEUHISPROGLUVAL
9   THRALATHRLEULYSVALHISVALTHRGLU
10   GLNLYS

Samples:

sample_1: CTL9, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 2.0; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15884 17460
PDB
EMBL CAA43972
GB AAA22701 AKM20686 AKU26730 KFL16938 KFX34457
PRF 0701226A 1714237A
REF WP_033008865 WP_050368025
SP P02417