BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15868

Title: 1H, 13C and 15N resonance assignment of guanidine-denatured GED of dynamin   PubMed: 17910478

Authors: Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna V

Citation: Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna V. "Pockets of short-range transient order and restricted topological heterogeneity in the guanidine-denatured state ensemble of GED of dynamin"  Biochemistry 46, 11819-11832 (2007).

Assembly members:
GED, polymer, 138 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GED: GSASFLRAGVYPERVGDKEK ASETEENGSDSFMHSMDPQL ERQVETIRNLVDSYMAIVNK TVRDLMPKTIMHLMINNTKE FIFSELLANLYSCGDQNTLM EESAEQAQRRDEMLRMYHAL KEALSIIGNINTTTVSTP

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts131
1H chemical shifts569

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GED1

Entities:

Entity 1, GED 138 residues - Formula weight is not available

1   GLYSERALASERPHELEUARGALAGLYVAL
2   TYRPROGLUARGVALGLYASPLYSGLULYS
3   ALASERGLUTHRGLUGLUASNGLYSERASP
4   SERPHEMETHISSERMETASPPROGLNLEU
5   GLUARGGLNVALGLUTHRILEARGASNLEU
6   VALASPSERTYRMETALAILEVALASNLYS
7   THRVALARGASPLEUMETPROLYSTHRILE
8   METHISLEUMETILEASNASNTHRLYSGLU
9   PHEILEPHESERGLULEULEUALAASNLEU
10   TYRSERCYSGLYASPGLNASNTHRLEUMET
11   GLUGLUSERALAGLUGLNALAGLNARGARG
12   ASPGLUMETLEUARGMETTYRHISALALEU
13   LYSGLUALALEUSERILEILEGLYASNILE
14   ASNTHRTHRTHRVALSERTHRPRO

Samples:

15N_GED: GED, [U-100% 15N], 1 mM; DTT 1 mM; sodium acetate 10 mM; sodium chloride 150 mM; guanidine 6 M; EDTA 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

15N_13C_GED: GED, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium acetate 10 mM; sodium chloride 150 mM; guanidine 6 M; EDTA 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 5.0; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_GEDisotropicsample_conditions_1
3D CBCA(CO)NH15N_13C_GEDisotropicsample_conditions_1
3D CBCANH15N_13C_GEDisotropicsample_conditions_1
3D HNCO15N_13C_GEDisotropicsample_conditions_1
3D HN(CA)CO15N_13C_GEDisotropicsample_conditions_1
3D 1H-15N NOESY15N_GEDisotropicsample_conditions_1
3D 1H-15N TOCSY15N_GEDisotropicsample_conditions_1
3D HNN15N_13C_GEDisotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15867 16948
PDB
DBJ BAD90284 BAE25726 BAG53342 BAG58137 BAG59018
EMBL CAA38397
GB AAA02803 AAA37318 AAA37319 AAA37323 AAA37324
PIR B40671
PRF 1614348A
REF NP_001005336 NP_001070288 NP_001124521 NP_001275666 NP_001275667
SP P21575 P39053 Q05193 Q08DF4
TPG DAA24145