BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15473

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for 8M Urea Denatured State of dSUMO-3(dSMT3)   PubMed: 19636913

Authors: Kumar, Dinesh; Misra, Jyoti; Chugh, Jeetender; Sharma, Shilpy

Citation: Kumar, Dinesh; Kumar, Ashutosh; Misra, Jyoti Ranjan; Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna. "1H, 15N, 13C resonance assignment of folded and 8 M urea-denatured state of SUMO from Drosophila melanogaster"  Biomol. NMR Assignments 2, 13-15 (2008).

Assembly members:
dSUMO, polymer, 90 residues, 9972.2 Da.

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
dSUMO: GSMSDEKKGGETEHINLKVL GQDNAVVQFKIKKHTPLRKL MNAYCDRAGLSMQVVRFRFD GQPINENDTPTSLEMEEGDT IEVYQQQTGG

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts90
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dSUMO1

Entities:

Entity 1, dSUMO 90 residues - 9972.2 Da.

1   GLYSERMETSERASPGLULYSLYSGLYGLY
2   GLUTHRGLUHISILEASNLEULYSVALLEU
3   GLYGLNASPASNALAVALVALGLNPHELYS
4   ILELYSLYSHISTHRPROLEUARGLYSLEU
5   METASNALATYRCYSASPARGALAGLYLEU
6   SERMETGLNVALVALARGPHEARGPHEASP
7   GLYGLNPROILEASNGLUASNASPTHRPRO
8   THRSERLEUGLUMETGLUGLUGLYASPTHR
9   ILEGLUVALTYRGLNGLNGLNTHRGLYGLY

Samples:

sample_1: dSUMO, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: pH: 5.7; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(C)Nsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

FELIX v2002, Accelrys Software Inc. - processing

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CBA18485
GB AAD19219 AAF31702 AAF52470 AAL28638 ALC38481
REF NP_001303312 NP_477411 XP_001355784 XP_001650444 XP_001962391