BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15409

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the cytoplasmic domain of the T cell receptor zeta chain   PubMed: 18311971

Authors: Jaravine, Victor; Zhuravleva, Anastasia; Permi, Perttu; Ibraghimov, Ilgis; Orekhov, Vladislav

Citation: Jaravine, Victor; Zhuravleva, Anastasia; Permi, Perttu; Ibraghimov, Ilgis; Orekhov, Vladislav. "Hyperdimensional NMR Spectroscopy with Nonlinear Sampling."  J. Am. Chem. Soc. 130, 3927-3936 (2008).

Assembly members:
T-cell_receptor_zeta-chain, polymer, 115 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
T-cell_receptor_zeta-chain: GSLRVKFSRSADAPAYQQGQ NQLYNELNLGRREEYDVLDK RRGRDPEMGGKPRRKNPQEG LYNELQKDKMAEAYSEIGMK GERRRGKGHDGLYQGLSTAT KDTYDALHMQALPPR

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 115 residues - Formula weight is not available

1   GLYSERLEUARGVALLYSPHESERARGSER
2   ALAASPALAPROALATYRGLNGLNGLYGLN
3   ASNGLNLEUTYRASNGLULEUASNLEUGLY
4   ARGARGGLUGLUTYRASPVALLEUASPLYS
5   ARGARGGLYARGASPPROGLUMETGLYGLY
6   LYSPROARGARGLYSASNPROGLNGLUGLY
7   LEUTYRASNGLULEUGLNLYSASPLYSMET
8   ALAGLUALATYRSERGLUILEGLYMETLYS
9   GLYGLUARGARGARGGLYLYSGLYHISASP
10   GLYLEUTYRGLNGLYLEUSERTHRALATHR
11   LYSASPTHRTYRASPALALEUHISMETGLN
12   ALALEUPROPROARG

Samples:

sample_1: T-cell receptor zeta-chain, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; Phosphate buffer 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.7; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D iHNCAsample_1isotropicsample_conditions_1
3D iHN(CA)CBsample_1isotropicsample_conditions_1

Software:

NMRPipe v2.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

MDD v1.3, (MDDnmr) V.Orekhov, V.Jaravine - data analysis, processing

NMR spectrometers:

  • Varian INOVA 900 MHz

Related Database Links:

DBJ BAC87407 BAD97045 BAG36664 BAJ20574
GB AAA60394 AAD34640 AAF34793 AAH25703 AAY57330
REF NP_000725 NP_932170 XP_001174731 XP_003258862 XP_003824647
SP P20963 Q9XSJ9