BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15398

Title: NH, NN, C and CA chemical shift assignment of the nuclear coactivator binding domain of CBP in the free state   PubMed: 18177052

Authors: Ebert, Marc-Olivier; Bae, Sung-Hun; Dyson, H.; Wright, Peter

Citation: Ebert, Marc-Olivier; Bae, Sung-Hun; Dyson, H.; Wright, Peter. "NMR Relaxation Study of the Complex Formed Between CBP and the Activation Domain of the Nuclear Hormone Receptor Coactivator ACTR"  Biochemistry 47, 1299-1308 (2008).

Assembly members:
CBP, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CBP: PNRSISPSALQDLLRTLKSP SSPQQQQQVLNILKSNPQLM AAFIKQRTAKYVANQPGMQ

Data sets:
Data typeCount
13C chemical shifts105
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP1

Entities:

Entity 1, CBP 59 residues - Formula weight is not available

1   PROASNARGSERILESERPROSERALALEU
2   GLNASPLEULEUARGTHRLEULYSSERPRO
3   SERSERPROGLNGLNGLNGLNGLNVALLEU
4   ASNILELEULYSSERASNPROGLNLEUMET
5   ALAALAPHEILELYSGLNARGTHRALALYS
6   TYRVALALAASNGLNPROGLYMETGLN

Samples:

sample_1: CBP, [U-98% 13C; U-98% 15N], 1 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.7; pressure: 1 atm; temperature: 304.15 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (HCA)CO(CA)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 16363 17073
PDB
DBJ BAE06125 BAI45616
GB AAB28651 AAC17736 AAC51331 AAC51340 AAC51770
PRF 1923401A
REF NP_001020603 NP_001073315 NP_001247644 NP_004371 NP_596872
SP P45481 Q6JHU9 Q92793