BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15397

Title: NH, NN, C, CA and CB chemical shift assignment of the transcriptional activation domain 1 of the nuclear hormone receptor coactivator ACTR in the free state   PubMed: 18177052

Authors: Ebert, Marc-Olivier; Bae, Sung-Hun; Dyson, H.; Wright, Peter

Citation: Ebert, Marc-Olivier; Bae, Sung-Hun; Dyson, H.; Wright, Peter. "NMR Relaxation Study of the Complex Formed Between CBP and the Activation Domain of the Nuclear Hormone Receptor Coactivator ACTR"  Biochemistry 47, 1299-1308 (2008).

Assembly members:
ACTR, polymer, 71 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ACTR: GTQNRPLLRNSLDDLVGPPS NLEGQSDERALLDQLHTLLS NTDATGLEEIDRALGIPELV NQGQALEPKQD

Data sets:
Data typeCount
13C chemical shifts182
15N chemical shifts62
1H chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACTR1

Entities:

Entity 1, ACTR 71 residues - Formula weight is not available

1   GLYTHRGLNASNARGPROLEULEUARGASN
2   SERLEUASPASPLEUVALGLYPROPROSER
3   ASNLEUGLUGLYGLNSERASPGLUARGALA
4   LEULEUASPGLNLEUHISTHRLEULEUSER
5   ASNTHRASPALATHRGLYLEUGLUGLUILE
6   ASPARGALALEUGLYILEPROGLULEUVAL
7   ASNGLNGLYGLNALALEUGLUPROLYSGLN
8   ASP

Samples:

sample_1: ACTR, [U-98% 13C; U-98% 15N], 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.7; pressure: 1 atm; temperature: 304.15 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D (HCA)CO(CA)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAD93100 BAF82372 BAG10971 BAG63847
GB AAB92368 AAC51663 AAC51677 AAC51849 AAH92516
REF NP_001107748 NP_001167558 NP_001167559 NP_006525 NP_858045
SP Q9Y6Q9
TPG DAA23082