BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15380

Title: Crystal Polymorphism of GB1 Examined by Solid-State NMR Spectroscopy and X-ray Diffraction   PubMed: 18052145

Authors: Frericks-Schmidt, Heather; Sperling, Lindsay; Gao, YiGui; Wylie, Benjamin; Boettcher, John; Wilson, Scott; Rienstra, Chad

Citation: Frericks-Schmidt, Heather; Sperling, Lindsay; Gao, YiGui; Wylie, Benjamin; Boettcher, John; Wilson, Scott; Rienstra, Chad. "Crystal Polymorphism of Protein GB1 Examined by Solid-State NMR Spectroscopy and X-ray Diffraction"  J. Phys. Chem. B 111, 14362-14369 (2007).

Assembly members:
GB1, polymer, 56 residues, 6900 Da.

Natural source:   Common Name: Staphylococcus sp. group G   Taxonomy ID: 1320   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus group G

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GB1: MQYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTE

Data typeCount
13C chemical shifts483
15N chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB11

Entities:

Entity 1, GB1 56 residues - 6900 Da.

1   METGLNTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

formulation_D: GB1, [U-100% 13C; U-100% 15N], 15 mg

formulation_E: GB1, [U-99% 13C; U-99% 15N], 17 mg

sample_conditions_1: pressure: 1 atm; temperature controller setting: 273 K

Experiments:

NameSampleSample stateSample conditions
2D N(CA)CXformulation_Dsolidsample_conditions_1
2D N(CO)CXformulation_Dsolidsample_conditions_1
3D NCACXformulation_Dsolidsample_conditions_1
3D NCOCXformulation_Dsolidsample_conditions_1
2D CC DARR mixingformulation_Dsolidsample_conditions_1
2D CC SPC5 mixingformulation_Dsolidsample_conditions_1
2D N(CA)CXformulation_Esolidsample_conditions_1
2D N(CO)CXformulation_Esolidsample_conditions_1
3D NCACXformulation_Esolidsample_conditions_1
3D NCOCXformulation_Esolidsample_conditions_1
2D CC DARR mixingformulation_Esolidsample_conditions_1
2D CC SPC5 mixingformulation_Esolidsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian InfinityPlus 500 MHz

Related Database Links:

UNP P06654