BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15379

Title: Sup35 NM   PubMed: 17767153

Authors: Toyama, Brandon; Kelly, Mark; Gross, John; Weissman, Jonathan

Citation: Toyama, Brandon; Kelly, Mark; Gross, John; Weissman, Jonathan. "The structural basis of yeast prion strain variants"  Nature 449, 233-237 (2007).

Assembly members:
Sup35-NM, polymer, 259 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sup35-NM: MSDSNQGNNQQNYQQYSQNG NQQQGNNRYQGYQAYNAQAQ PAGGYYQNYQGYSGYQQGGY QQYNPDAGYQQQYNPQGGYQ QYNPQGGYQQQFNPQGGRGN YKNFNYNNNLQGYQAGFQPQ SQGMSLNDFQKQQKQAAPKP KKTLKLVSSSGIKLANATKK VGTKPAESDKKEEEKSAETK EPTKEPTKVEEPVKKEEKPV QTEEKTEEKSELPKVEDLKI SESTHNTNNANVTSADALIK EQEEEVDDEVVNDHHHHHH

Data sets:
Data typeCount
15N chemical shifts132
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sup35 NM domain1

Entities:

Entity 1, Sup35 NM domain 259 residues - Formula weight is not available

1   METSERASPSERASNGLNGLYASNASNGLN
2   GLNASNTYRGLNGLNTYRSERGLNASNGLY
3   ASNGLNGLNGLNGLYASNASNARGTYRGLN
4   GLYTYRGLNALATYRASNALAGLNALAGLN
5   PROALAGLYGLYTYRTYRGLNASNTYRGLN
6   GLYTYRSERGLYTYRGLNGLNGLYGLYTYR
7   GLNGLNTYRASNPROASPALAGLYTYRGLN
8   GLNGLNTYRASNPROGLNGLYGLYTYRGLN
9   GLNTYRASNPROGLNGLYGLYTYRGLNGLN
10   GLNPHEASNPROGLNGLYGLYARGGLYASN
11   TYRLYSASNPHEASNTYRASNASNASNLEU
12   GLNGLYTYRGLNALAGLYPHEGLNPROGLN
13   SERGLNGLYMETSERLEUASNASPPHEGLN
14   LYSGLNGLNLYSGLNALAALAPROLYSPRO
15   LYSLYSTHRLEULYSLEUVALSERSERSER
16   GLYILELYSLEUALAASNALATHRLYSLYS
17   VALGLYTHRLYSPROALAGLUSERASPLYS
18   LYSGLUGLUGLULYSSERALAGLUTHRLYS
19   GLUPROTHRLYSGLUPROTHRLYSVALGLU
20   GLUPROVALLYSLYSGLUGLULYSPROVAL
21   GLNTHRGLUGLULYSTHRGLUGLULYSSER
22   GLULEUPROLYSVALGLUASPLEULYSILE
23   SERGLUSERTHRHISASNTHRASNASNALA
24   ASNVALTHRSERALAASPALALEUILELYS
25   GLUGLNGLUGLUGLUVALASPASPGLUVAL
26   VALASNASPHISHISHISHISHISHIS

Samples:

sample_1: Sup35-NM, [U-15N], 100-300 uM; DMSO 95%; H2O 4.5%; dichloroacetic acid-d2 0.5%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
HN(CA)NHsample_1isotropicsample_conditions_1
CT-HNCAsample_1isotropicsample_conditions_1
HN(CA)COsample_1isotropicsample_conditions_1

Software:

ANSIG v3.3, Kraulis - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 18406 18407
DBJ GAA22402
EMBL CAA30155 CAA68760 CAA86677 CAY78675
GB AAA35133 AAF99684 AAK26176 AAK26180 AAK26182
REF NP_010457
SP P05453
TPG DAA12014