BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15226

Title: High resolution Solid state NMR structure of Kaliotoxin bound to Potassium channel   PubMed: 18523586

Authors: Korukottu, Jegannath; Lange, Adam; Vijayan, Vinesh; Schneider, Robert; Pongs, Olaf; Becker, Stefan; Baldus, Marc; Zweckstetter, Markus

Citation: Korukottu, Jegannath; Schneider, Robert; Vijayan, Vinesh; Lange, Adam; Pongs, Olaf; Becker, Stefan; Baldus, Marc; Zweckstetter, Markus. "High-resolution 3D structure determination of kaliotoxin by solid-state NMR spectroscopy."  PLoS ONE 3, e2359-. (2008).

Assembly members:
subunit 1, polymer, 38 residues, 4168.079 Da.

Natural source:   Common Name: Androctonus mauretanicus   Taxonomy ID: 6859   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Androctonus mauretanicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
subunit 1: GVEINVKCSGSPQCLKPCKD AGMRFGKCMNRKCHCTPK

Data sets:
Data typeCount
13C chemical shifts100
15N chemical shifts23

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 38 residues - 4168.079 Da.

1   GLYVALGLUILEASNVALLYSCYSSERGLY
2   SERPROGLNCYSLEULYSPROCYSLYSASP
3   ALAGLYMETARGPHEGLYLYSCYSMETASN
4   ARGLYSCYSHISCYSTHRPROLYS

Samples:

sample_1: entity, [U-13C; U-15N], 2 mM

sample_conditions_1: ionic strength: 2 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NHHCsample_1isotropicsample_conditions_1
CHHCsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAB20997
SP P24662