BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15225

Title: 1H, 13C, and 15N resonance assignments of domain 2 of non-structural protein 5A (NS5A) of hepatitis C virus   PubMed: 17880107

Authors: Liang, Yu; Ye, Hong; Kang, Cong Bao; Yoon, Ho Sup

Citation: Liang, Yu; Ye, Hong; Kang, Cong Bao; Yoon, Ho Sup. "Domain 2 of Non-structural Protein 5A (NS5A) of Hepatitis C Virus is Natively Unfolded"  Biochemistry 46, 11550-11558 (2007).

Assembly members:
Domain_2_of_Non-structural_Protein_5A_(NS5AD2), polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: hepatitis c virus   Taxonomy ID: 11103   Superkingdom: not available   Kingdom: Viruses   Genus/species: not available Hepacivirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Domain_2_of_Non-structural_Protein_5A_(NS5AD2): KATCTANHDSPDAELIEANL LWRQEMGGNITRVESENKVV ILDSFDPLVAEEDEREVSVP AEILRKSRRFARALPVWARP DYNPPLVETWKKPDYEHHHH HH

Data sets:
Data typeCount
13C chemical shifts385
15N chemical shifts93
1H chemical shifts636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Domain 2 of Non-structural Protein 5A (NS5AD2)1

Entities:

Entity 1, Domain 2 of Non-structural Protein 5A (NS5AD2) 102 residues - Formula weight is not available

1   LYSALATHRCYSTHRALAASNHISASPSER
2   PROASPALAGLULEUILEGLUALAASNLEU
3   LEUTRPARGGLNGLUMETGLYGLYASNILE
4   THRARGVALGLUSERGLUASNLYSVALVAL
5   ILELEUASPSERPHEASPPROLEUVALALA
6   GLUGLUASPGLUARGGLUVALSERVALPRO
7   ALAGLUILELEUARGLYSSERARGARGPHE
8   ALAARGALALEUPROVALTRPALAARGPRO
9   ASPTYRASNPROPROLEUVALGLUTHRTRP
10   LYSLYSPROASPTYRGLUHISHISHISHIS
11   HISHIS

Samples:

sample_1: Domain 2 of Non-structural Protein 5A (NS5AD2), [U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.01%

sample_2: Domain 2 of Non-structural Protein 5A (NS5AD2), [U-13C; U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

FELIX v2004, Accelrys Software Inc. - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

EMBL CAJ20207 CAJ20220
GB AAA45534 AAA45676 AAA45677 AAB66324 AAB67036
REF NP_671491 NP_751927
SP P26664 P27958