BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15138

Title: 13C and 15N resonance assignments for the major coat protein in Pf1 filamentous bacteriophage   PubMed: 17279748

Authors: Goldbourt, Amir; Gross, Benjamin; Day, Loren; McDermott, Ann

Citation: Goldbourt, Amir; Gross, Benjamin; Day, Loren; McDermott, Ann. "Filamentous phage studied by magic-angle spinning NMR: resonance assignment and secondary structure of the coat protein in Pf1."  J. Am. Chem. Soc. 129, 2338-2344 (2007).

Assembly members:
Pf1_phage_coat_protein, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: Purified from host after deliberate infection   Host organism: Pseudomonas aeruginosa

Entity Sequences (FASTA):
Pf1_phage_coat_protein: GVIDTSAVESAITDGQGDMK AIGGYIVGALVILAVAGLIY SMLRKA

Data typeCount
13C chemical shifts196
15N chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Coat protein1

Entities:

Entity 1, Coat protein 46 residues - Formula weight is not available

This is the major coat protein of Pf1 filamentous bacteriophage. Also referred to as gene-8 protein product, g8p.

1   GLYVALILEASPTHRSERALAVALGLUSER
2   ALAILETHRASPGLYGLNGLYASPMETLYS
3   ALAILEGLYGLYTYRILEVALGLYALALEU
4   VALILELEUALAVALALAGLYLEUILETYR
5   SERMETLEUARGLYSALA

Samples:

Pf1_U: Pf1 phage, [U-13C; U-15N], 7 – 10 mg

Pf1_NCO: Pf1 phage, [U-15N; 13C C1-Glucose], 7 – 10 mg

sample_conditions_1: pH: 8.4; temperature: 297.5 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXPf1_UPrecipitated solidsample_conditions_1
3D NCOCXPf1_UPrecipitated solidsample_conditions_1
2D CC DARRPf1_UPrecipitated solidsample_conditions_1
3D NCOCXPf1_NCOPrecipitated solidsample_conditions_1
2D NN PDSDPf1_NCOPrecipitated solidsample_conditions_1
3D NCACXPf1_UPrecipitated solidsample_conditions_1
3D NCOCXPf1_UPrecipitated solidsample_conditions_1
2D CC DARRPf1_UPrecipitated solidsample_conditions_1
2D CC DARRPf1_UPrecipitated solidsample_conditions_1

Software:

SPARKY v3.110, Goddard - chemical shift assignment, peak picking

NMRPipe v2.3 Rev 2004.184.22.03, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Varian InfinityPlus 600 MHz
  • Varian InfinityPlus 400 MHz

Related Database Links:

NCBI Taxonomy ID 10871
SWS P03621
ATCC 25102-B1
GB x52107 AAG04112 AAT50608 ACS36651 AGV62109 AGV64452
PDB
DBJ BAQ41860
EMBL CAA36331 CCQ85809 CDH73426 CDO83853 CFU90266
PRF 754922A
REF NP_039603 NP_249414 NP_955532 WP_003125072 WP_023090795
SP P03621