BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15136

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Endosulfine alpha   PubMed: 19636856

Authors: Boettcher, John; Hartman, Kevin; Ladror, Daniel; Qi, Zhi; Woods, Wendy; Rienstra, Chad; George, Julia

Citation: Boettcher, John; Hartman, Kevin; Ladror, Daniel; Qi, Zhi; Woods, Wendy; George, Julia; Rienstra, Chad. "(1)H, (13)C, and (15)N resonance assignment of the cAMP-regulated phosphoprotein endosulfine-alpha in free and micelle-bound states"  Biomol. NMR Assignments 1, 167-169 (2007).

Assembly members:
ENSA, polymer, 121 residues, 13.389 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ENSA: MSQKQEEENPAEETGEEKQD TQEKEGILPERAEEAKLKAK YPSLGQKPGGSDFLMKRLQK GQKYFDSGDYNMAKAKMKNK QLPSAGPDKNLVTGDHIPTP QDLPQRKSSLVTSKLAGGQV E

Data sets:
Data typeCount
13C chemical shifts304
15N chemical shifts96
1H chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ENSA, chain 11
2ENSA, chain 21

Entities:

Entity 1, ENSA, chain 1 121 residues - 13.389 Da.

1   METSERGLNLYSGLNGLUGLUGLUASNPRO
2   ALAGLUGLUTHRGLYGLUGLULYSGLNASP
3   THRGLNGLULYSGLUGLYILELEUPROGLU
4   ARGALAGLUGLUALALYSLEULYSALALYS
5   TYRPROSERLEUGLYGLNLYSPROGLYGLY
6   SERASPPHELEUMETLYSARGLEUGLNLYS
7   GLYGLNLYSTYRPHEASPSERGLYASPTYR
8   ASNMETALALYSALALYSMETLYSASNLYS
9   GLNLEUPROSERALAGLYPROASPLYSASN
10   LEUVALTHRGLYASPHISILEPROTHRPRO
11   GLNASPLEUPROGLNARGLYSSERSERLEU
12   VALTHRSERLYSLEUALAGLYGLYGLNVAL
13   GLU

Samples:

sample_1: sodium phosphate 50 mM; sodium azide 0.02%; ENSA, [U-100% 13C; U-100% 15N], 0.5 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

SPARKY, Goddard - data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15135
DBJ BAB24433 BAB27552 BAB31584 BAE29049 BAE36298
EMBL CAA06798 CAA06799 CAA06800 CAA06801 CAA65196
GB AAD32454 AAF80340 AAF80341 AAH00436 AAH04461
REF NP_001021383 NP_001029146 NP_001300934 NP_004427 NP_062507
SP O43768 P60840 P60841 P68210 P68211
TPG DAA31642