BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11461

Title: One-disulfide variant of hen lysozyme: 1SS[64-80]   PubMed: 22344587

Authors: Noda, Yasuo; Narama, Kuniaki; Kasai, Kenichi; Murakami, Shusaku; Tachibana, Hideki; Segawa, Shin-ichi

Citation: Noda, Yasuo; Narama, Kuniaki; Kasai, Kenichi; Tachibana, Hideki; Segawa, Shin-Ichi. "Glycerol-enhanced detection of a preferential structure latent in unstructured 1SS-variants of lysozyme."  Biopolymers 97, 539-549 (2012).

Assembly members:
1SS[64-80], polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
1SS[64-80]: MKVFGRSELAAAMKRHGLDN YRGYSLGNWVAAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLANIP CSALLSSDITASVNAAKKIV SDGNGMNAWVAWRNRAKGTD VQAWIRGARL

Data sets:
Data typeCount
15N chemical shifts124
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
11SS[64-80]1

Entities:

Entity 1, 1SS[64-80] 130 residues - Formula weight is not available

This sample sequence have Met containing the N-terminal residue, because it is produced in Escherichia coli.

1   METLYSVALPHEGLYARGSERGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   ALAALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPCYSASNASPGLYARGTHR
8   PROGLYSERARGASNLEUALAASNILEPRO
9   CYSSERALALEULEUSERSERASPILETHR
10   ALASERVALASNALAALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGALALYSGLYTHRASP
13   VALGLNALATRPILEARGGLYALAARGLEU

Samples:

sample_1: 1SS[64-80], [U-99% 15N], 1 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%

sample_conditions_1: pH*: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N HSQC-NOESY-HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 11051 11052 11459 11460 11462 15198 18365 18366 18367 18368 18369 18370
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