BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11052

Title: Two-disulfide variant of hen lysozyme: 2SS[6-127, 64-80]   PubMed: 19353641

Authors: Sakamoto, Keiko; Hirai, Ken-ichi; Kitamura, Yoshiaki; Yamazaki, Kouta; Yusa, Mitsunobu; Tokunaga, Naoki; Doi, Gakuji; Noda, Yasuo; Tachibana, Hideki; Segawa, Shin-ichi

Citation: Sakamoto, Keiko; Hirai, Ken-ichi; Kitamura, Yoshiaki; Yamazaki, Kouta; Yusa, Mitsunobu; Tokunaga, Naoki; Doi, Gakuji; Noda, Yasuo; Tachibana, Hideki; Segawa, Shin-Ichi. "Glycerol-induced folding of unstructured disulfide-deficient lysozyme into a native-like conformation"  Biopolymers 91, 665-675 (2009).

Assembly members:
2SS(6-127_64-80), polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
2SS(6-127_64-80): MKVFGRCELAAAMKRHGLDN YRGYSLGNWVAAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLANIP CSALLSSDITASVNAAKKIV SDGNGMNAWVAWRNRAKGTD VQAWIRGCRL

Data sets:
Data typeCount
15N chemical shifts123
1H chemical shifts517

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12SS(6-127_64-80)1

Entities:

Entity 1, 2SS(6-127_64-80) 130 residues - Formula weight is not available

1   METLYSVALPHEGLYARGCYSGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   ALAALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPCYSASNASPGLYARGTHR
8   PROGLYSERARGASNLEUALAASNILEPRO
9   CYSSERALALEULEUSERSERASPILETHR
10   ALASERVALASNALAALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGALALYSGLYTHRASP
13   VALGLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: 2SS(6-127_64-80), [U-99% 15N], 1 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%

sample_2: 2SS(6-127_64-80), [U-99% 15N], 0.2 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%

sample_conditions_1: pH*: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N HSQC-NOESY-HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 1093 11051 11459 11461 15198 18304 18305 18365 4562
PDB
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .