BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11051

Title: Disulfide-free variant of hen lysozyme: 0SS   PubMed: 19353641

Authors: Sakamoto, Keiko; Hirai, Ken-ichi; Kitamura, Yoshiaki; Yamazaki, Kouta; Yusa, Mitsunobu; Tokunaga, Naoki; Doi, Gakuji; Noda, Yasuo; Tachibana, Hideki; Segawa, Shin-ichi

Citation: Sakamoto, Keiko; Hirai, Ken-ichi; Kitamura, Yoshiaki; Yamazaki, Kouta; Yusa, Mitsunobu; Tokunaga, Naoki; Doi, Gakuji; Noda, Yasuo; Tachibana, Hideki; Segawa, Shin-Ichi. "Glycerol-induced folding of unstructured disulfide-deficient lysozyme into a native-like conformation"  Biopolymers 91, 665-675 (2009).

Assembly members:
0SS-variant, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
0SS-variant: MKVFGRSELAAAMKRHGLDN YRGYSLGNWVAAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWANDGRTPGSRNLANIP ASALLSSDITASVNAAKKIV SDGNGMNAWVAWRNRAKGTD VQAWIRGARL

Data sets:
Data typeCount
15N chemical shifts125
1H chemical shifts536

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
10SS-variant1

Entities:

Entity 1, 0SS-variant 130 residues - Formula weight is not available

1   METLYSVALPHEGLYARGSERGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   ALAALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPALAASNASPGLYARGTHR
8   PROGLYSERARGASNLEUALAASNILEPRO
9   ALASERALALEULEUSERSERASPILETHR
10   ALASERVALASNALAALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGALALYSGLYTHRASP
13   VALGLNALATRPILEARGGLYALAARGLEU

Samples:

sample_1: 0SS-variant, [U-99% 15N], 1 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%

sample_2: 0SS-variant, [U-99% 15N], 0.2 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%

sample_conditions_1: pH*: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N HSQC-NOESY-HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 11052 11459 11460 11461 11462 15198 18365 18366 18367 18368 18369 18370