BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11028

Title: Solid-state NMR assignment of the rigid core of the HET-s(218-289) prion protein in its amyloid conformation.   PubMed: 18339938

Authors: Siemer, Ansgar; Wasmer, Christian; Lange, Adam; Van Melckebeke, Helene; Ernst, Matthias; Ritter, Christiane; Steinmetz, Michel; Riek, Roland; Meier, Beat

Citation: Wasmer, Christian; Lange, Adam; Van Melckebeke, Helene; Siemer, Ansgar; Riek, Roland; Meier, Beat. "Amyloid fibrils of the HET-s(218-289) prion form a beta-solenoid with a triangular hydrophobic core."  Science 319, 1523-1526 (2008).

Assembly members:
HET-s(218-289), polymer, 79 residues, 8667.732 Da.

Natural source:   Common Name: Podospora anserina   Taxonomy ID: 5145   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Podospora anserina

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HET-s(218-289): MKIDAIVGRNSAKDIRTEER ARVQLGNVVTAAALHGGIRI SDQTTNSVETVVGKGESRVL IGNEYGGKGFWDNHHHHHH

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HET-s(218-289)1

Entities:

Entity 1, HET-s(218-289) 79 residues - 8667.732 Da.

1   METLYSILEASPALAILEVALGLYARGASN
2   SERALALYSASPILEARGTHRGLUGLUARG
3   ALAARGVALGLNLEUGLYASNVALVALTHR
4   ALAALAALALEUHISGLYGLYILEARGILE
5   SERASPGLNTHRTHRASNSERVALGLUTHR
6   VALVALGLYLYSGLYGLUSERARGVALLEU
7   ILEGLYASNGLUTYRGLYGLYLYSGLYPHE
8   TRPASPASNHISHISHISHISHISHIS

Samples:

sample_1: HET-s(218-289), [U-100% 13C; U-100% 15N], 5 – 40 mg; H2O%

sample_conditions_1: ionic strength: 0 M; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
DREAMsample_1solidsample_conditions_1
TOBSYsample_1solidsample_conditions_1
PDSDsample_1solidsample_conditions_1
NCAsample_1solidsample_conditions_1
NCOsample_1solidsample_conditions_1
N(CO)CAsample_1solidsample_conditions_1
N(CO)CBsample_1solidsample_conditions_1
N(CA)COsample_1solidsample_conditions_1
CA-CAsample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

SPARKY, Goddard - data analysis

CARA, Rochus Keller - data analysis

xwinnmr, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz

Related Database Links:

BMRB 11064
PDB
GB AAB19707 AAB94631
PRF 1718317A
SP Q03689